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. 2021;57(8):845-850.
doi: 10.1134/S0003683821080032. Epub 2021 Dec 14.

Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

J A Makarova  1 , K A Fomicheva  2 , A I Osipyants  2   3 , M Yu Shkurnikov  2 , A A Pokryshchenko  1 , E A Tonevitsky  4 , V I Vechorko  5
Affiliations

Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

J A Makarova et al. Appl Biochem Microbiol. 2021.

Abstract

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.

Keywords: COVID-19; LAMP; SARS-CoV-2; coronavirus; diagnostics; loop-mediated isothermal amplification.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Scheme of LAMP primers: F3—forward outer primer; В3—backward outer primer; FIP—forward inner primer; BIP—backward inner primer; LFP—loop forward primer, LBP—loop backward primer
See this image and copyright information in PMC

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