Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Mar 20;235(4795):1504-8.
doi: 10.1126/science.3493529.

Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein

G G Wong, P A Temple, A C Leary, J S Witek-Giannotti, Y C Yang, A B Ciarletta, M Chung, P Murtha, R Kriz, R J Kaufman, et al.

Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein

G G Wong et al. Science. .

Abstract

A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.

PubMed Disclaimer