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. 2021 Dec 5;10(12):1491.
doi: 10.3390/antibiotics10121491.

Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume

Ton That Huu Dat  1 , Phung Thi Thuy Oanh  1 , Le Canh Viet Cuong  1 , Le Tuan Anh  1 , Le Thi Hong Minh  2 , Hoang Ha  3 , Le Tung Lam  3 , Pham Viet Cuong  1 , Hoang Le Tuan Anh  4
Affiliations

Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume

Ton That Huu Dat et al. Antibiotics (Basel). .

Abstract

Mangrove plant endophytic bacteria are prolific sources of bioactive secondary metabolites. In the present study, twenty-three endophytic bacteria were isolated from the fresh roots of the mangrove plant Rhizophora apiculata. The identification of isolates by 16S rRNA gene sequences revealed that the isolated endophytic bacteria belonged to nine genera, including Streptomyces, Bacillus, Pseudovibrio, Microbacterium, Brevibacterium, Microbulbifer, Micrococcus, Rossellomorea, and Paracoccus. The ethyl acetate extracts of the endophytic bacteria's pharmacological properties were evaluated in vitro, including antimicrobial, antioxidant, α-amylase and α-glucosidase inhibitory, xanthine oxidase inhibitory, and cytotoxic activities. Gas chromatography-mass spectrometry (GC-MS) analyses of three high bioactive strains Bacillus sp. RAR_GA_16, Rossellomorea vietnamensis RAR_WA_32, and Bacillus sp. RAR_M1_44 identified major volatile organic compounds (VOCs) in their ethyl acetate extracts. Genome analyses identified biosynthesis gene clusters (BGCs) of secondary metabolites of the bacterial endophytes. The obtained results reveal that the endophytic bacteria from R. apiculata may be a potential source of pharmacological secondary metabolites, and further investigations of the high bioactive strains-such as fermentation and isolation of pure bioactive compounds, and heterologous expression of novel BGCs in appropriate expression hosts-may allow exploring and exploiting the promising bioactive compounds for future drug development.

Keywords: GC-MS; Rhizophora apiculata; biological activity; biosynthesis gene clusters; endophytic bacteria; genome.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Rhizophora apiculata collected from the Bu Lu mangrove forest, Phu Loc district, Thua Thien Hue province, Vietnam.
Figure 2
Figure 2
Phylogeny of 16S rRNA gene sequences of the endophytic bacteria in our study (bold letters) and from the National Center for Biotechnology Information (NCBI) GenBank. Bootstrap support values of branches greater than 75% are given above the corresponding branches.
Figure 3
Figure 3
Similarity of predicted BGCs from genomes and the MIBiG database.
See this image and copyright information in PMC

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