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. 2021 Dec 3;11(12):3452.
doi: 10.3390/ani11123452.

The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters

Uchechi Linda Ohaneje  1 , Uchebuchi Ike Osuagwuh  1   2 , Manuel Alvarez-Rodríguez  3   4 , Iván Yánez-Ortiz  1 , Abigail Tabarez  5 , María Jesús Palomo  1
Affiliations

The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters

Uchechi Linda Ohaneje et al. Animals (Basel). .

Abstract

In order to achieve a higher post-thaw buck sperm quality, an approach in the thawing protocol of cryopreserved sperm doses under in vitro capacitation conditions mimicking the in vivo female environment was studied. Therefore, functional and kinetic characteristics of buck thawed sperm from males of different ages, the season of collection, and melatonin implanted males in the non-breeding season were assessed after 3 h of incubation in an in vitro fertilization (IVF) media with 20% of buck seminal plasma (SP). Previously, fresh ejaculates were collected via artificial vagina from eight males of the Cabra Blanca de Rasquera breed during two consecutive years in breeding and non-breeding periods. Prior to semen collection in non-breeding seasons, males were split into two groups: one group was implanted with melatonin, while the other was not. In each group, semen samples were pooled, centrifuged, and diluted in an extender containing 15% powdered egg yolk and 5% glycerol before freezing. After thawing, sperm were washed and incubated in three different media: (a) control media (modified phosphate-buffered saline (PBS), (b) IVF commercial media, and (c) IVF media + 20% SP. Sperm motility was evaluated by CASA, while plasma and acrosome membrane integrity, mitochondria activity, and DNA fragmentation were analysed by flow cytometer at 0 h and after 3 h incubation. A significant reduction in motility, mitochondrial activity, plasma, and acrosome membrane integrity were observed after incubation in the presence of SP, although similar to that observed in IVF media alone. DNA integrity was not affected under in vitro capacitation conditions, regardless of SP addition. In conclusion, the addition of SP failed to improve post-thaw buck sperm quality under in vitro conditions irrespective of male age, the season of collection, and melatonin implant.

Keywords: age; buck sperm; cryopreservation; incubation; melatonin; season; seminal plasma.

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Conflict of interest statement

The authors declare no conflict of interest.

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References

    1. Dorado J., Rodríguez I., Hidalgo M. Cryopreservation of Goat Spermatozoa: Comparison of Two Freezing Extenders Based on Post-Thaw Sperm Quality and Fertility Rates after Artificial Insemination. Theriogenology. 2007;68:168–177. doi: 10.1016/j.theriogenology.2007.04.048. - DOI - PubMed
    1. Ntemka A., Tsakmakidis I.A., Kiossis E., Milovanović A., Boscos C.M. Current status and advances in ram semen cryopreservation. J. Hellenic Vet. Med. Soc. 2018;69:911–924. doi: 10.12681/jhvms.18014. - DOI
    1. Purdy P.H. A Review on Goat Sperm Cryopreservation. Small Rumin. Res. 2006;63:215–225. doi: 10.1016/j.smallrumres.2005.02.015. - DOI
    1. Roy A. Egg Yolk-Coagulating Enzyme in the Semen and Cowper’s Gland of the Goat. Nature. 1957;179:318–319. doi: 10.1038/179318b0. - DOI - PubMed
    1. Gangwar C., Kharche S.D., Kumar S., Jindal S.K. Cryopreservation of Goat Semen: Status and Prospects. Indian J. Small Rumin. 2016;22:1–10. doi: 10.5958/0973-9718.2016.00005.2. - DOI