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. 2021 Dec 19;11(12):3594.
doi: 10.3390/ani11123594.

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Petr Slama  1 , Terezie Zavadilova  1 , Ales Pavlik  1 , Pavel Horky  2 , Sylvie Skalickova  2 , Jiri Skladanka  2 , Shubhadeep Roychoudhury  3 , Simona Baldovska  4 , Adriana Kolesarova  5 , Roman Konecny  6 , Vladimir Tancin  7   8 , Monika Zouharova  9
Affiliations

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Petr Slama et al. Animals (Basel). .

Abstract

In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.

Keywords: Streptococcus uberis; T cell; apoptosis; lymphocyte; mammary gland; mastitis.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
The proportion of lymphocytes following induction of bovine mammary glands with PBS (control) and S. uberis. Statistically significant differences between control (PBS) and S. uberis are marked with asterisks (** p < 0.01).
Figure 2
Figure 2
The proportion of γδ T cells following the induction of bovine mammary gland with PBS (control) and S. uberis. Statistically significant differences between the control (PBS) and S. uberis are marked with asterisks (** p < 0.01).
Figure 3
Figure 3
The proportion of apoptotic lymphocytes following the induction of bovine mammary glands with PBS (control) and S. uberis. Statistically significant differences between control (PBS) and S. uberis are marked with asterisks (** p < 0.01).
See this image and copyright information in PMC

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