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. 2021 Dec 8;12(12):1523.
doi: 10.3390/mi12121523.

Construction of Photoelectrochemical DNA Biosensors Based on TiO2@Carbon Dots@Black Phosphorous Quantum Dots

Kai Song  1 , Jianwei Lin  1   2 , Yafeng Zhuang  1   2 , Zhizhong Han  1   2 , Jinghua Chen  1   2
Affiliations

Construction of Photoelectrochemical DNA Biosensors Based on TiO2@Carbon Dots@Black Phosphorous Quantum Dots

Kai Song et al. Micromachines (Basel). .

Abstract

In this work, carbon dots (CDs) and black phosphorus quantum dots (BPQDs) were used to decorate titanium dioxide to enhance the photoelectrochemical (PEC) properties of the nanocomposites (TiO2@CDs@BPQDs), and the modified nanocomposites were used to sensitively detect DNA. We used the hydrothermal method and citric acid as a raw material to prepare CDs with good dispersion and strong fluorescence properties. BPQDs with a uniform particle size were prepared from black phosphorus crystals. The nanocomposites were characterized by fluorescence spectroscopy, UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The preparation method of the working electrode was explored, the detection conditions were optimized, and the sensitive detection of target DNA was achieved. The results demonstrate that CDs and BPQDs with good optical properties were successfully prepared, and they were successfully combined with TiO2 to improve the PEC performance of TiO2@CDs@BPQDs. The TiO2-based PEC DNA detection method was constructed with a detection limit of 8.39 nM. The constructed detection method has many advantages, including good sensitivity, a wide detection range, and good specificity. This work provides a promising PEC strategy for the detection of other biomolecules.

Keywords: black phosphorus quantum dots; carbon dots; photoelectrochemical biosensor; titanium dioxide.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Scheme 1
Scheme 1
Schematic constructed process of the biosensors based on TCPs nanocomposites.
Figure 1
Figure 1
(A) Transmission electron microscopy (TEM) image, (B) UV-Vis absorption spectrum and fluorescence spectrum, and (C) Fourier transform infrared spectrum (FTIR) of carbon dots (CDs).
Figure 2
Figure 2
(A,B) TEM image, (C) UV-Vis absorption spectrum and fluorescence spectrum, (D) FTIR of BPQDs.
Figure 3
Figure 3
(A) Photocurrent of the electrodes prepared with different concentrations of TiO2, (B) fluorescence emission spectra, and (C) Photoelectrochemical (PEC) properties for different samples.
Figure 4
Figure 4
(A) PEC characterization, (B,C) CV curves of the different electrodes.
Figure 5
Figure 5
Factors affecting the PEC performance of the constructed electrode, (A) incubation time, and (B) pH value.
Figure 6
Figure 6
(A) Photocurrent and (B) linear fitting of the constructed electrode after adding different concentrations of T-DNA. (C) Photocurrent comparison after adding different DNA.
See this image and copyright information in PMC

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