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. 2021 Dec 20;10(12):3155.
doi: 10.3390/foods10123155.

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Lijia Zhang  1 , Mengzhou Yin  1 , Xi Feng  2 , Salam A Ibrahim  3 , Ying Liu  1 , Wen Huang  1
Affiliations

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Lijia Zhang et al. Foods. .

Abstract

In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

Keywords: Poriae Cutis; anti-inflammatory; identification; purification; triterpenoid.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Elution profile of triterpenoids by HSCCC chromatogram. n-hexane-ethyl acetate-MeOH-water (3:6:4:2, v/v/v/v); flow rate: 3 mL/min; detection wavelength: 242 nm; rotational speed: 800 rpm; sample size: 100 mg.
Figure 2
Figure 2
HPLC chromatogram of four kinds of triterpenoids obtained by HSCCC.
Figure 3
Figure 3
The structures of compounds 1–4.
Figure 4
Figure 4
The effects of various concentrations of poricoic acid A and poricoic acid B (A), dehydrotrametenolic acid and dehydroeburicoic acid (B) on the cell viabilities of murine macrophage cells (RAW 264.7). The values are expressed as the mean ± SD (n = 3). Different letters (a–g) indicate significant differences (p < 0.05). LPS, lipopolysaccharide.
Figure 5
Figure 5
The effects of various concentrations of poricoic acid A and poricoic acid B (A), dehydrotrametenolic acid and dehydroeburicoic acid (B) on the NO production in lipopolysaccharide (LPS)-stimulated murine macrophage cells (RAW 264.7). The values are presented as the mean ± SD (n = 3). Different letters (a–f) indicate significant differences (p < 0.05).
Figure 6
Figure 6
The effect of various concentrations of poricoic acid B on the productions of TNF-α (A), IL-1β (B), and IL-6 (C) in lipopolysaccharide (LPS)-stimulated murine macrophage cells (RAW 264.7). The values are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01 vs. LPS group.
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