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. 2021 Dec;12(2):12702-12721.
doi: 10.1080/21655979.2021.2009977.

The nephroprotective effects of Daucus carota and Eclipta prostrata against cisplatin-induced nephrotoxicity in rats

Muhammad Omer Iqbal  1 , Asad Saleem Sial  2 , Imran Akhtar  3 , Muhammad Naeem  4 , Abu Hazafa  5 , Rais A Ansari  6 , Syed A A Rizvi  7
Affiliations

The nephroprotective effects of Daucus carota and Eclipta prostrata against cisplatin-induced nephrotoxicity in rats

Muhammad Omer Iqbal et al. Bioengineered. 2021 Dec.

Abstract

The overuse of cisplatin (>50 mg/m2) is limited to nephrotoxicity, ototoxicity, gastrotoxicity, myelosuppression, and allergic reactions. The objective of this study was to investigate the nephroprotective effects of Daucus carota and Eclipta prostrata extracts on cisplatin-induced nephrotoxicity in Wistar albino rats. The study involved male Wistar albino rats of 8 weeks weighing 220-270 g. A single injection of 5 mg/kg was injected into the rats for nephrotoxicity. Rats were divided into four groups based on dose conentrations. Blood and urine samples of rats were collected on the 0, 7th, 14th, and 21st days for nephrological analysis. The results showed that Cis + DC/Cis + EP (600 mg/kg) significantly (p < 0.001) increased the body weight and reduced the kidney weight of cisplatin-induced nephrotoxicity in rats (p < 0.001) as compared to Cis group. The results showed that 600 mg/kg administration of Cis + DC/Cis +EP successfully (p < 0.005) improved the urine and plasmin creatinine, Na, and K level compared to the Cis group. Histopathological results confirmed that Cis + EP/Cis + DC effectively improved the renal abnormalities. It is concluded that the co-administration of Cis + EP extract showed exceptional nephroprotective effects at a dose rate of 600 mg/kg.

Keywords: Cisplatin; Daucus carota; Eclipta prostrata; nephroprotection; nephrotoxicity; rats.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Effect of (a) DC and (b) EP extracts on kidney weight in cisplatin-treated rats. Where Cis is cisplatin (5 mg/kg i.p), Cis + DCa is cisplatin + Daucus carota extract (400 mg/kg/21 days), Cis + DCb is cisplatin + Daucus carota extract (600 mg/kg/21 days. Cis + EPa is cisplatin + Eclipta prostrata extract (400 mg/kg/21 days) and Cis + EPb is cisplatin + Eclipta prostrata extract (600 mg/kg/21 days). *p < 0.001 and **p < 0.05
Figure 2.
Figure 2.
Representation of effect of (a) DC and (b) EP extracts on body urine output in cisplatin-treated rats. The results showed that co-administration of Cis + DC/Cis + EP significantly reduced the body urine output at a dose administration of 600 mg/kg. *p < 0.001, **p < 0.05, and ***p < 0.005
Figure 3.
Figure 3.
Histogram of the effect of (a) DC and (b) EP extracts on urinary sodium in cisplatin-treated rats. The results showed that co-administration of Cis + DC/Cis + EP significantly reduced the body urinary Na at the dose administration of 600 mg/kg. *p < 0.001, **p < 0.05
Figure 4.
Figure 4.
Effect of (a) DC and (b) EP plant extracts on urinary potassium in cisplatin-induced nephrotoxic rats. *p < 0.001, **p < 0.05, and ***p < 0.005
Figure 5.
Figure 5.
Representation of effect of (a) DC and (b) EP plant extracts on plasmin sodium in cisplatin-induced nephrotoxic rats. *p < 0.001, **p < 0.05, and ***p < 0.005
Figure 6.
Figure 6.
Histogram of the effect of (a) DC and (b) EP extracts on plasmin potassium in cisplatin-treated rats. The results showed that co-administration of Cis + DC/Cis + EP significantly reduced the body urine output at dose administration of 600 mg/kg. *p < 0.005, **p < 0.05, and ns = non-significant
Figure 7.
Figure 7.
The representation of (a) DC and (b) EP plant extracts on urinary creatinine in cisplatin-induced nephrotoxic rats. *p < 0.001, ** p < 0.05, and ***p < 0.005
Figure 8.
Figure 8.
Interpretation of effect of (a) DC and (b) EP plant extracts on plasmin creatinine in cisplatin-induced nephrotoxic rats. *p < 0.001, ** p < 0.05, and ***p < 0.005
Figure 9.
Figure 9.
Photomicrograph of histopathological analysis of (a) control, (b) Cis group, (c) Cis + EP (400 mg/kg), and (d) Cis + EP (600 mg/kg)
Figure 10.
Figure 10.
Photomicrograph of kidney tissues of rats for (a) control, (b) Cis group, (c) Cis + DC (400 mg/kg), and (d) Cis + DC (600 mg/kg)
See this image and copyright information in PMC

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