Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Kenneth W Witwer¹, Deborah Ci Goberdhan², Lorraine O'Driscoll³, Clotilde Théry⁴, Joshua A Welsh⁵, Cherie Blenkiron⁶, Edit I Buzás⁷, Dolores Di Vizio⁸, Uta Erdbrügger⁹, Juan M Falcón-Pérez¹⁰, Qing-Ling Fu¹¹, Andrew F Hill¹², Metka Lenassi¹³, Jan Lötvall¹⁴, Rienk Nieuwland¹⁵, Takahiro Ochiya¹⁶, Sophie Rome¹⁷, Susmita Sahoo¹⁸, Lei Zheng¹⁹

Affiliations

¹ Departments of Molecular and Comparative Pathobiology and Neurology and The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
² Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
³ School of Pharmacy and Pharmaceutical Sciences, Panoz Institute and Trinity Biomedical Sciences, Institute (TBSI) & Trinity St. James's Cancer Institute (TSJCI), Trinity College Dublin, Dublin, Ireland.
⁴ INSERM U932, Institut Curie, PSL Research University, Paris, France.
⁵ Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
⁶ Department of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
⁷ Department of Genetics, Cell- and Immunobiology, HCEMM-SU Extracellular Vesicles Research Group, and ELKH-SE Immune-Proteogenomics Extracellular Vesicles Research Group, Semmelweis University, Budapest, Hungary.
⁸ Department of Surgery, Department of Pathology & Laboratory Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Division of Cancer Biology and Therapeutics, Los Angeles, California, USA.
⁹ Department of Medicine, Division of Nephrology, University of Virginia, Charlottesville, Virginia, USA.
¹⁰ Exosomes Laboratory & Metabolomics Platf, CIC bioGUNE-BRTA, IKERBASQUE, CIBERehd, Bilbao, Spain.
¹¹ Exosome Research and Translational Center, The First Affiliated Hospital, Otorhinolaryngology Hospital, Sun Yat-sen University, Guangzhou, China.
¹² Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia.
¹³ Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
¹⁴ Krefting Research Centre, University of Gothenburg, Göteborg, Sweden.
¹⁵ Laboratory of Experimental Clinical Chemistry and Vesicle Observation Center, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
¹⁶ Department of Molecular and Cellular Medicine, Tokyo Medical University, Tokyo, Japan.
¹⁷ CarMeN Laboratory (INSERM 1060, INRAE 1397), University of Lyon & Faculty of Medicine Lyon-Sud, Pierre-Bénite, France.
¹⁸ Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
¹⁹ Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

PMID: 34953156
PMCID: PMC8710080
DOI: 10.1002/jev2.12182

Editorial

Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Kenneth W Witwer et al. J Extracell Vesicles. 2021 Dec.

. 2021 Dec;10(14):e12182.

doi: 10.1002/jev2.12182.

Authors

Affiliations

¹ Departments of Molecular and Comparative Pathobiology and Neurology and The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
² Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
³ School of Pharmacy and Pharmaceutical Sciences, Panoz Institute and Trinity Biomedical Sciences, Institute (TBSI) & Trinity St. James's Cancer Institute (TSJCI), Trinity College Dublin, Dublin, Ireland.
⁴ INSERM U932, Institut Curie, PSL Research University, Paris, France.
⁵ Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
⁶ Department of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
⁷ Department of Genetics, Cell- and Immunobiology, HCEMM-SU Extracellular Vesicles Research Group, and ELKH-SE Immune-Proteogenomics Extracellular Vesicles Research Group, Semmelweis University, Budapest, Hungary.
⁸ Department of Surgery, Department of Pathology & Laboratory Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Division of Cancer Biology and Therapeutics, Los Angeles, California, USA.
⁹ Department of Medicine, Division of Nephrology, University of Virginia, Charlottesville, Virginia, USA.
¹⁰ Exosomes Laboratory & Metabolomics Platf, CIC bioGUNE-BRTA, IKERBASQUE, CIBERehd, Bilbao, Spain.
¹¹ Exosome Research and Translational Center, The First Affiliated Hospital, Otorhinolaryngology Hospital, Sun Yat-sen University, Guangzhou, China.
¹² Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia.
¹³ Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
¹⁴ Krefting Research Centre, University of Gothenburg, Göteborg, Sweden.
¹⁵ Laboratory of Experimental Clinical Chemistry and Vesicle Observation Center, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
¹⁶ Department of Molecular and Cellular Medicine, Tokyo Medical University, Tokyo, Japan.
¹⁷ CarMeN Laboratory (INSERM 1060, INRAE 1397), University of Lyon & Faculty of Medicine Lyon-Sud, Pierre-Bénite, France.
¹⁸ Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
¹⁹ Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

PMID: 34953156
PMCID: PMC8710080
DOI: 10.1002/jev2.12182

Abstract

The minimal information for studies of extracellular vesicles (EVs, MISEV) is a field-consensus rigour initiative of the International Society for Extracellular Vesicles (ISEV). The last update to MISEV, MISEV2018, was informed by input from more than 400 scientists and made recommendations in the six broad topics of EV nomenclature, sample collection and pre-processing, EV separation and concentration, characterization, functional studies, and reporting requirements/exceptions. To gather opinions on MISEV and ideas for new updates, the ISEV Board of Directors canvassed previous MISEV authors and society members. Here, we share conclusions that are relevant to the ongoing evolution of the MISEV initiative and other ISEV rigour and standardization efforts.

Keywords: MISEV; ectosomes; exosomes; extracellular vesicles; microvesicles; reproducibility; rigor; standardization.

PubMed Disclaimer

Conflict of interest statement

EIB: Advisory board of Sphere Gene Therapeutics Inc.; JL: owns stock in Codiak BioSciences; ownership in Exocure biosciences Inc.; consults in the EV field through Vesiclebio AB; DCIG: Consultancy agreement with Codiak BioSciences.

Figures

**FIGURE 1**
Status and EV experience of MISEV survey respondents. All questions were answered by all 764 respondents unless indicated in parentheses in the title of the respective panel

**FIGURE 2**
Respondent engagement with MISEV. All questions were answered by all 764 respondents unless indicated in parentheses in the title of the respective panel

**FIGURE 3**
ISEV2018 sections to retain and topics to add. The total number of respondents for each question is indicated in parentheses in each panel title and by the vertical orange line; exact number of responses for each option is shown within each bar. WB, western blot; EM, electron microscopy

See this image and copyright information in PMC

References

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Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Affiliations

Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources