High N2O reduction potential by denitrification in the nearshore site of a riparian zone

Abstract

As a potent atmospheric greenhouse gas and a major source of ozone depletion, nitrous oxide (N₂O) emission has been given increasing attention in aquatic systems, particularly at the aquatic-terrestrial interfaces, such as riparian zones. However, the microbial mechanisms regulating N₂O emission in riparian zones remain unknown. Here, we measured the contributions of denitrification and ammonium oxidation to N₂O emission along with the abundance and community structure of nirK-, nirS-, nosZ I- and nosZ II-harbouring bacteria in both surface sediments (0-10 cm) and overlying water along a lake riparian zone (including nearshore sites and offshore sites). Overall, the nearshore sites of the riparian zones emitted less N₂O than the offshore sites. Nearshore N₂O emission was dominated by denitrification with a high N₂O reduction rate, whereas offshore N₂O emission was driven by ammonium oxidation. Furthermore, N₂O derived from ammonium oxidation was influenced by the NH₄⁺-N content, and denitrification N₂O was modulated by denitrifier communities. The N₂O-producing community was dominated by nirS-harbouring bacteria, while the N₂O-reducing community was dominated by nosZ I-harbouring bacteria. The relative abundance of Hydrogenophilales from nirS-denitrifiers and Chloroflexi unclassified from nosZ II-type communities influenced the N₂O produced by denitrification, according to high-throughput sequencing analysis. Additionally, we also found lower levels of N₂O production per unit volume in overlying water, which were 3-4 orders of magnitude less than in the surface sediment. Overall, we propose that using riparian zones can be an effective management tool for N₂O mitigation by enhancing the N₂O reduction process of denitrification and decreasing ammonium oxidation.

Keywords: Ammonium oxidation; Denitrification; N(2)O-producers; N(2)O-reducers; Nitrous oxide; Riparian zone.