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. 2022 Mar 26;14(1):mjab081.
doi: 10.1093/jmcb/mjab081.

NuMA forms condensates through phase separation to drive spindle pole assembly

Huixian Ma  1 , Feifei Qi  1 , Li Ji  1 , Songbo Xie  1 , Jie Ran  1 , Min Liu  1 , Jinmin Gao  1 , Jun Zhou  1   2
Affiliations

NuMA forms condensates through phase separation to drive spindle pole assembly

Huixian Ma et al. J Mol Cell Biol. .

Erratum in

  • Erratum.
    [No authors listed] [No authors listed] J Mol Cell Biol. 2022 May 25;14(2):mjac030. doi: 10.1093/jmcb/mjac030. J Mol Cell Biol. 2022. PMID: 35639540 Free PMC article. No abstract available.
No abstract available

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Figures

Figure 1
Figure 1
Phase separation of NuMA promotes spindle pole formation. (A) Schematic representations of full-length (FL) NuMA and truncated mutants. (B) Fluorescence images showing the localization of GFP-tagged full-length NuMA and truncated mutants in HeLa cells. Scale bar, 5 μm. (C and D) Immunofluorescence images of mitotic spindles (C) and quantification of the percentage of mitotic cells with multipolar spindles (D, n = 3 independent experiments) for HeLa cells transfected with the indicated siRNAs and plasmids. For each experiment, 60 mitotic cells were quantified. Scale bar, 5 μm. (E and F) FRAP analysis (E) and quantification (F, n = 2 independent experiments) of GFP-NuMA signals in HeLa cells. Scale bar, 2 μm. (G and H) Representative images (G) and size (H, n = 100 droplets from three independent experiments) of droplets formed by purified GFP-NuMA-C wild type (WT) or proline-to-alanine (P/A) mutant. Scale bar, 10 μm. (I) Time-lapse microscopy showing the fusion of GFP-NuMA-C droplets into a larger droplet. Scale bar, 10 μm. (J and K) FRAP analysis (J) and quantification (K, n = 3 independent experiments) of droplets formed by 10 μM purified GFP-NuMA-C. Scale bar, 5 μm. (L) Fluorescence images showing the localization of GFP-NuMA-C wild type and mutant in metaphase HeLa cells. Scale bar, 5 μm. (M and N) Immunofluorescence images of mitotic spindles (M) and quantification of the percentage of mitotic cells with multipolar spindles (N, n = 4 independent experiments) for HeLa cells transfected as indicated. For each experiment, 60 mitotic cells were quantified. Scale bar, 5 μm. Data are shown as mean ± SD. ***P < 0.001, ****P <0.0001.
See this image and copyright information in PMC

References

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