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. 2022 May;22(9):e2100031.
doi: 10.1002/pmic.202100031. Epub 2022 Jan 5.

BLI-MS: Combining biolayer interferometry and mass spectrometry

Vincent Jung  1 , Kévin Roger  1 , Cerina Chhuon  1 , Louise Pannetier  2 , Joanna Lipecka  1 , Josué Sulub Gomez  1 , Pascal Chappert  2   3 , Alain Charbit  2 , Ida Chiara Guerrera  1
Affiliations

BLI-MS: Combining biolayer interferometry and mass spectrometry

Vincent Jung et al. Proteomics. 2022 May.

Abstract

Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures.

Keywords: biolayer interferometry; mass spectrometry; molecular interaction.

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References

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