Rice organs concentrate cadmium by chelation of amino acids containing dicarboxyl groups and enhance risks to human and environmental health in Cd-contaminated areas

Abstract

When rice plants grown in paddy fields with Cd content of 0.3-1.5 mg kg^-1, Cd quantities in roots and straws were 2-7 times higher than that in topsoil. Return of these vegetative organs to topsoil aggravated the ecological risk of Cd pollution. Cd content in rice grains was 0.1-1.3 mg kg^-1, and hazard quotients for local consumers by intake of these rice were 0.7-8.8. Planting low-Cd-accumulating (LCA) cultivar reduced hazard quotients for consumers by intake of rice, but had similar ecological risks as high-accumulating (HCA) cultivars. LCA cultivar had lower Cd content in grains as well as higher efficiency of altering Cd into insoluble forms in flag leaves and upmost nodes than HCA cultivars. Insoluble Cd content in nodes was linearly increased with soil Cd content, companied by significant decline of 4 amino acids with dicarboxyl groups. Glu or Asp can form a cyclic complex with Cd by two O atoms from α-COO- and side chain-COO-. These results indicate that roots and straws have high potential to concentrate Cd by forming complexes between amino acids and Cd ions, and Cd-enriched straw return to topsoil may aggravate the ecological risk of Cd contamination.

Keywords: Cadmium; Ecological risk; Glutamate; Insoluble forms; Rice.