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. 2021 Dec 3;10(12):2658.
doi: 10.3390/plants10122658.

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Fatma Zohra Chakou  1   2 , Zakaria Boual  1 , Mohamed Didi Ould El Hadj  1 , Hakim Belkhalfa  3 , Khaldoun Bachari  3 , Zainab El Alaoui-Talibi  4 , Cherkaoui El Modafar  4 , Farah Hadjkacem  1   5 , Imen Fendri  6 , Slim Abdelkafi  5 , Mounir Traïkia  7 , Didier Le Cerf  8 , Pascal Dubessay  2 , Cédric Delattre  2 , Guillaume Pierre  2 , Philippe Michaud  2
Affiliations

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Fatma Zohra Chakou et al. Plants (Basel). .

Abstract

The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagi maurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, 1H/13C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 106 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.

Keywords: Alhagi; Sahara; galactomannan; polysaccharides.

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Conflict of interest statement

No conflict of interest to declare.

Figures

Figure 1
Figure 1
FT-IR spectrum of WSPAM1.
Figure 2
Figure 2
Monosaccharide compositions of WSPAM1 (a) and WSPAM2 (b) using GC-MS/EI.
Figure 3
Figure 3
Spectra 1H NMR (a) and 13C NMR (b) of WSPAM1 from Alhagi maurorum Medik. seeds. G: α-d-galactopyranose; M: β-d-mannopyranose non substituted; M’: β-d-mannopyranose substituted at O-6 position.
Figure 4
Figure 4
Inhibitory effects of WSPAM1, WSPAM2 and Ibuprofen on BSA thermally denaturation. + p > 0.1 and ++ p < 0.01 compared to Ibuprofen. Correlation between the inhibitory effect of polysaccharide extracts and the molecular weight was expressed with +++ p < 0.001.
Figure 5
Figure 5
Antihyperglycemic activity of polysaccharide extracts of A. maurorum Medik. seeds on inhibition of α-amylase enzyme activity. ✶ p < 0.001, ✶✶ p < 0.05, ✶✶✶ p < 0.1 compared with acarbose (positive control) as well as to to molecular weight (🗴 p < 0.0001).
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