Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov;67(6):475-484.
doi: 10.18097/PBMC20216706475.

[The bioinformatic identification of proteins with varying levels of post-translational modifications in experimental ischemic stroke in mice]

[Article in Russian]
V S Skvortsov  1 , Ya O Ivanova  1 , A I Voronina  1
Affiliations

[The bioinformatic identification of proteins with varying levels of post-translational modifications in experimental ischemic stroke in mice]

[Article in Russian]
V S Skvortsov et al. Biomed Khim. 2021 Nov.

Abstract
in English, Russian

The experimental data obtained by Simats A. et al. (Molecular and Cellular Proteomics, 2020, 19(12), 1921-1936) was analysed using a bioinformatic approach. Original experimental results available in the ProteomeXchange database were obtained using a comprehensive multidomain approach to identify potential blood biomarkers in ischemic stroke in mice. The identification of peptides with post-translational modification (PTM) was performed by us using the raw data (accession code PXD016538). Only phosphorylation and deamination were considered as PTMs. Different combinations of data sets (ischemic tissue with intact tissue, ischemic tissue with control taken from mice after sham surgery, etc.) were compared both in terms of the ratio of abundance for the modified peptide to the unmodified variant and in terms of absolute values of abundance. The most likely change in precisely PTM levels was shown for 27 proteins, which include dynamin, glycogen phosphorylase and 70 kDa heat shock protein.

Proanalizirovany éksperimental'nye dannye (Simats A. et al. (2020) Molecular and Cellular Proteomics, 19(12), 1921-1936), poluchennye pri ispol'zovanii kompleksnogo mul'tiomnogo podkhoda po vyiavleniiu potentsial'nykh biomarkerov krovi pri ishemicheskom insul'te u mysheĭ, dostupnye v baze dannykh ProteomeXchange (accession code PXD016538). Identifikatsiia peptidov s posttransliatsionnymi modifikatsiia (PTM) byla provedena zanovo. V kachestve PTM rassmatrivali tol'ko fosforilirovanie i dezaminirovanie. Razlichnye kombinatsii dannykh (ishemicheskaia tkan' s nepovrezhdennoĭ, ishemicheskaia tkan' s kontrolem, vziatym u mysheĭ posle mnimoĭ operatsii, i t.d.) sravnivali kak po otnosheniiu predstavlennosti (abundance) dlia modifitsirovannogo peptida k nemodifitsirovannomu variantu, tak i po absoliutnym znacheniiam predstavlennosti. Naibolee veroiatnoe izmenenie urovnia PTM pokazano dlia 27 belkov, k chislu kotorykh otnosiatsia dinamin, glikogenfosforilaza i belok teplovogo shoka 70 kDa.

Keywords: bioinformatics; ischemic stroke; posttranslational modifications.

PubMed Disclaimer

LinkOut - more resources