Microglia sense neuronal activity via GABA in the early postnatal hippocampus

Abstract

Microglia, the resident macrophages in the central nervous system, express receptors for classical neurotransmitters, such as γ-aminobutyric acid (GABA) and glutamate, suggesting that they sense synaptic activity. To detect microglial Ca²⁺ responses to neuronal activity, we generate transgenic mouse lines expressing the fluorescent Ca²⁺ indicator GCaMP6m, specifically in microglia and demonstrate that electrical stimulation of the Schaffer collateral pathway results in microglial Ca²⁺ responses in early postnatal but not adult hippocampus. Preceding the microglial responses, we also observe similar Ca²⁺ responses in astrocytes, and both are sensitive to tetrodotoxin. Blocking astrocytic glutamate uptake or GABA transport abolishes stimulation-induced microglial responses as well as antagonizing the microglial GABA_B receptor. Our data, therefore, suggest that the neuronal activity-induced glutamate uptake and the release of GABA by astrocytes trigger the activation of GABA_B receptors in microglia. This neuron, astrocyte, and microglia communication pathway might modulate microglial activity in developing neuronal networks.

Keywords: GABA; astrocyte; calcium; development; glutamate; hippocampus; microglia; neuron; neurotransmitter receptors; transporters.