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. 2021 Dec 20:2021:9919129.
doi: 10.1155/2021/9919129. eCollection 2021.

Influence of N6-Methyladenosine Modification Gene HNRNPC on Cell Phenotype in Parkinson's Disease

Wei Quan  1 , Jia Li  1 , Li Liu  1 , Qinghui Zhang  1 , Yidan Qin  1 , Xiaochen Pei  1 , Jiajun Chen  1
Affiliations

Influence of N6-Methyladenosine Modification Gene HNRNPC on Cell Phenotype in Parkinson's Disease

Wei Quan et al. Parkinsons Dis. .

Abstract

This study aimed to explore the N6-methyladenosine (m6A) modification genes involved in the pathogenesis of Parkinson's disease (PD) through data analysis of the two data sets GSE120306 and GSE22491 in the GEO database and further explore its influence on cell phenotype in PD. We analyzed the differentially expressed genes and function enrichment analysis of the two sets of data and found that the expression of the m6A-modification gene HNRNPC was significantly downregulated in the PD group, and it played an important role in DNA metabolism, RNA metabolism, and RNA processing and may be involved in PD. Then, we constructed the HNRNPC differential expression cell line to study the role of this gene in the pathogenesis of PD. The results showed that overexpression of HNRNPC can promote the proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory factors IFN-β, IL-6, and TNF-α, suggesting that HNRNPC may cause PD by inhibiting the proliferation of dopaminergic nerve cells, promoting their apoptosis, and causing immune inflammation. Our study also has certain limitations. For example, the data of the experimental group and the validation group come from different cell types, and the data of the experimental group involve individuals with G2019S LRRK2 mutations. In addition, due to the low expression of HNRNPC in PC12 cells, we used the method of overexpressing this gene to study its function. All these factors may cause our conclusions to be biased. Therefore, more research is still needed to corroborate it in the future.

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Conflict of interest statement

The authors declare that there are no conflicts of interest regarding the publication of this article.

Figures

Figure 1
Figure 1
Venn diagrams of DEGs in the experimental group and the verification group.
Figure 2
Figure 2
Bubble chart of GO-KEGG enrichment results. The more the genes in each enrichment item, the bigger the bubble, and the P value from high to low corresponds to the color from red to green.
Figure 3
Figure 3
GO analysis involving HNRNPC. The more the genes in each enrichment item, the larger the bubble, and the P value from high to low corresponds to the color from red to green.
Figure 4
Figure 4
The expression of HNRNPC gene in two sets of data sets.
Figure 5
Figure 5
The effect of overexpression of HNRNPC on the proliferation of PC12 cells. (a) Detection of HNRNPC mRNA expression in each group by qPCR. (b, c) Detection of HNRNPC protein expression in each group by western blotting. (d) Detection of cell proliferation ratio in each group by CCK-8. CON: control group, NC: empty plasmid group, HNRNPC+: overexpression HNRNPC group.
Figure 6
Figure 6
The effect of overexpression of HNRNPC on PC12 cell apoptosis. CON: control group, NC: empty plasmid group, HNRNPC+: overexpression HNRNPC group.
Figure 7
Figure 7
The effect of overexpression of HNRNPC on the expression of inflammatory factors in each group. CON: control group, NC: empty plasmid group, HNRNPC+: overexpression HNRNPC group.
Figure 8
Figure 8
The effect of overexpression of HNRNPC on autophagy in PC12 cells. The red arrow in the figure is the autophagosome. CON: control group, NC: empty plasmid group, HNRNPC+: overexpression HNRNPC group.
See this image and copyright information in PMC

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