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. 2021 Nov;501(1):419-423.
doi: 10.1134/S1607672921060053. Epub 2021 Dec 29.

Peroxide-Induced Damage to Plasminogen Molecules

A D Vasilyeva  1 , V S Ivanov  2 , L V Yurina  2 , M I Indeykina  2   3 , A E Bugrova  2 , A S Kononikhin  4 , E N Nikolaev  5 , M A Rosenfeld  2
Affiliations

Peroxide-Induced Damage to Plasminogen Molecules

A D Vasilyeva et al. Dokl Biochem Biophys. 2021 Nov.

Abstract

Plasminogen is a zymogenic form of plasmin, an enzyme that plays a fundamental role in the dissolution of fibrin clots as well as in many other physiological processes. For the first time, by the method of gas chromatography-mass spectrometry, post-translational modifications in the primary structure of plasminogen treated with physiologically relevant amounts of hydrogen peroxide were identified. It was found that methionine and tryptophan residues located in different structural regions of plasminogen served as targets of the oxidant. Plasminogen oxidation caused a dose-dependent effect in decreasing the fibrinogenolytic activity of plasmin evidenced by the formation of fibrinogen degradation products. The possible antioxidant role of methionines in the oxidative modification of plasminogen is discussed.

Keywords: antioxidant methionines; electrophoresis; hydrogen peroxide; mass spectrometry; oxidation; oxidation sites; plasminogen/plasmin.

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REFERENCES

    1. Murray, V., Norrving, B., Sandercock, P., et al., The molecular basis of thrombolysis and its clinical application in stroke, J. Intern. Med., 2010, vol. 267, pp. 191–208. - DOI
    1. Weisel, J.W., Fibrinogen and fibrin, Adv. Protein Chem., 2005, vol. 70, pp. 247–299. - DOI
    1. Castellino, F. and Ploplis, V., Structure and function of the plasminogen/plasmin system, Thromb. Haemost., 2005, vol. 93, pp. 647–654. - DOI
    1. Miles, L.A., Dahlberg, C.M., and Plow, E.F., The cell-binding domains of plasminogen and their function in plasma, J. Biol. Chem., 1988, vol. 263, pp. 11928–11934. - DOI
    1. Law, R.H., Caradoc-Davies, T., Cowieson, N., et al., The X-ray crystal structure of full-length human plasminogen, Cell Rep., 2012, vol. 1, pp. 185–190. - DOI

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