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Review
. 2023 Sep-Oct;42(5):1647-1687.
doi: 10.1002/mas.21768. Epub 2021 Dec 29.

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the "gold standard" method and integration into clinical practice

Anastasia Alexandridou  1 , Pascal Schorr  1 , Caroline S Stokes  2   3 , Dietrich A Volmer  1
Affiliations
Review

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the "gold standard" method and integration into clinical practice

Anastasia Alexandridou et al. Mass Spectrom Rev. 2023 Sep-Oct.

Abstract

Liquid chromatography/tandem mass spectrometry is firmly established today as the gold standard technique for analysis of vitamin D, both for vitamin D status assessments as well as for measuring complex and intricate vitamin D metabolic fingerprints. While the actual mass spectrometry technology has seen only incremental performance increases in recent years, there have been major, very impactful changes in the front- and back-end of MS-based vitamin D assays; for example, the extension to new types of biological sample matrices analyzed for an increasing number of different vitamin D metabolites, novel sample preparation techniques, new powerful chemical derivatization reagents, as well the continued integration of high resolution mass spectrometers into clinical laboratories, replacing established triple-quadrupole instruments. At the same time, the sustainability of mass spectrometry operation in the vitamin D field is now firmly established through proven analytical harmonization and standardization programs. The present review summarizes the most important of these recent developments.

Keywords: 25-hydroxyvitamin D3; HRMS; LC-MS/MS; chemical derivatization; epimers; free vitamin D; sample preparation; vitamin D metabolites; vitamin D standardization.

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References

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