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. 2021 Dec 1;22(12):3913-3919.
doi: 10.31557/APJCP.2021.22.12.3913.

Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia's Apis cerana: An In Vitro Study

Kenny Lischer  1 , Shania Rosita Angelica Sitorus  1 , Brian Wirawan Guslianto  1 , Forbes Avila  1 , Apriliana Cahya Khayrani  1 , Muhamad Sahlan  1
Affiliations

Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia's Apis cerana: An In Vitro Study

Kenny Lischer et al. Asian Pac J Cancer Prev. .

Abstract

Objective: Breast cancer is the most common case of cancers. Apitheraphy has been traditionally used for abundance diseases. This study aims to evaluate and compare the anti-breast cancer activity of melittin from Indonesia's Apic cerana as a potential drug for treating breast cancer.

Methods: Apis cerana bee venom (BV) was collected from a bee farm in Cikurutung, Bandung using an electrical venom device. The BV was then purified using the ÄKTA Start system and HiTrap™ SP HP cation exchange chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify melittin based on its molecular mass and lowry's protein assay to measure melittin concentration. Melittin cytotoxicity was measured with brine shrimp lethality test (BSLT), while MCF-7 breast cancer cells MTT assay was used to measure its anti-breast cancer activity, based on inhinition rate.

Results: 95.432 μg/mL melittin is purified from 62.8 mg/L BV, using cation exchange chromatography. Melittin in vitro analysis with MCF-7 MTT assay is used to determine anti-breast cancer activity in dose dependent manner. Furthermore, melttin BSLT result showed a LC50 16.67675 μg/mL. Therefore, the MTT assay was conducted in 5, 10 and 15 μg/mL with MCF-7 inhibition values of 0.768 ± 0.014, 3.303 ± 0.011, and 35.714 ± 0.009 %, respectively.

Conclusion: Indonesia's Apis cerana has the potential to be used as a therapeutic peptide for breast cancer treatment.

Keywords: Apis cerana; MCF-7 Cells; anti-cancer; melittin.

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Figures

Figure 1
Figure 1
Apris cerana Melittin Purification Profile from ÄKTA Start System UNICRON 1.1 Program Interface. Blue : UV, Red (dotted) : Main log, Red : Counductivity, Purple : Gradient concentration
Figure 2
Figure 2
BV to Melittin Purification SDS-PAGE Profile. M : Protein marker, BV : Crude venom, P1 : Peak 1, P2 : Peak 2, P3 : Peak 3, P4 : Peak 4, and P5 : Peak 5
Figure 3
Figure 3
MTT Asasy Comparison between Apis cerana BV & Melittin. (a) MTT assay using MCF-7 breast cancer cells cultured with the addition BV and melttin extract. The control condition for the MTT assay is when there is no BV or melittin added into the cultured. (b) The inhibition rate (%) of varied BV and melittin concentration (each condition are replicated three times) assay
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