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. 2022 Jan 1;26(1):65-68.
doi: 10.5588/ijtld.21.0478.

VIDAS® TB-IGRA reagents induce a CD4+ and CD8+ T-cell IFN-γ response for both TB infection and active TB

E Petruccioli  1 , C Farroni  1 , G Cuzzi  1 , V Vanini  2 , F Palmieri  3 , P Vittozzi  3 , D Goletti  1
Affiliations

VIDAS® TB-IGRA reagents induce a CD4+ and CD8+ T-cell IFN-γ response for both TB infection and active TB

E Petruccioli et al. Int J Tuberc Lung Dis. .
No abstract available

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Conflict of interest statement

Conflicts of interest: DG is a consultant of bioMérieux (Marcy l’Etoile, France), Qiagen (Hilden, Germany), and Diasorin and received a grant from Biomerieux. This study was supported by bioMérieux, and the Italian Ministry of Health (Ricerca Corrente-Linea 4).

Figures

Figure 1
Figure 1
Gating strategy for IFN-γ analysis in T-cell subsets and IFN-γ production by CD4+ and CD8+ T-cells in patients with active TB and LTBI. Plots show the distribution and frequency of IFN-γ in PBMCs of a representative active TB and LTBI subject. Lymphocytes were gated based on their SSC-A and FSC-A parameters, following which only live cells were considered according to the negativity of the Viability Stain antibody and single cells were gated as described. CD3+ T-cells were gated according to the expression of their surface marker. SSC-A = side scatter; FSC-A = forward scatter; IFN-γ = interferon-gamma; LTBI = latent TB infection; PBMC = peripheral blood mononuclear cell.
Figure 2
Figure 2
Percentage of IFN-γ+ CD4+ T-cells and IFN-γ+ CD8+ T-cells in untreated cells and in response to AG stimulation in LTBI subjects and active TB patients. LTBI = latent TB infection; AG = MTB antigen; IFN-γ = interferon-gamma; FSC-A = forward scatter.
Figure 3
Figure 3
Left: Box showing both numbers and percentage of participants who showed a CD4+ or/and CD8+ T-cell response. Middle and right graphs: percentage of CD4+, CD8+/IFN-γ+ T-cells in the responders to VIDAS TB-IGRA AG. Each dot/square represents a single active TB or LTBI patient, black lines represent median values. Individuals who showed both CD4+ and CD8+ responses are represented with the same colour in all graphs. Individuals who showed a single CD4+ or CD8+ T-cell response are indicated in white. No differences between groups were observed using the Mann–Whitney test. AG = (VIDAS TB-IGRA) antigen tube reagent; LTBI = latent TB infection; IFN-γ = interferon-gamma.
See this image and copyright information in PMC

References

    1. World Health Organization WHO; 2021. Global Tuberculosis Report 2021.https://www.who.int/publications/i/item/9789240037021
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