Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2460:115-125.
doi: 10.1007/978-1-0716-2148-6_7.

Synthetic Plant Glycan Microarrays as Tools for Plant Biology

Colin Ruprecht  1   2 , Fabian Pfrengle  3   4
Affiliations

Synthetic Plant Glycan Microarrays as Tools for Plant Biology

Colin Ruprecht et al. Methods Mol Biol. 2022.

Abstract

Chemically synthesized plant oligosaccharides have recently evolved as powerful molecular tools for plant cell wall biology. Synthetic plant glycan microarrays equipped with these oligosaccharides enable high-throughput analyses of glycan-binding proteins and carbohydrate-active enzymes. To produce these glycan microarrays, small amounts of glycan solution are printed on suitable surfaces for covalent or non-covalent immobilization. Synthetic plant glycan microarrays have been used for example to map the epitopes of plant cell wall-directed antibodies, to characterize glycosyl hydrolases and glycosyl transferases, and to analyze lectin binding. In this chapter, detailed experimental procedures for the production of synthetic glycan microarrays and their use for the characterization of cell wall glycan-directed antibodies are described.

Keywords: Cell wall glycan-directed antibodies; Glycan microarray; Glycan-binding proteins; Lectins; Plant cell wall oligosaccharides.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Varki A (2017) Biological roles of glycans. Glycobiology 27(1):3–49
    1. Fukui S et al (2002) Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions. Nat Biotechnol 20(10):1011–1017 - PubMed
    1. Park SJ, Shin IJ (2002) Fabrication of carbohydrate chips for studying protein-carbohydrate interactions. Angew Chem Int Ed Engl 41(17):3180 - PubMed
    1. Wang D et al (2002) Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells. Nat Biotechnol 20(3):275–281 - PubMed
    1. Blixt O et al (2004) Printed covalent glycan array for ligand profiling of diverse glycan binding proteins. Proc Natl Acad Sci U S A 101(49):17033–17038 - PubMed - PMC

Publication types

LinkOut - more resources