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. 2022:2460:183-191.
doi: 10.1007/978-1-0716-2148-6_11.

Detection of Autoantibodies Using Combinatorial Glycolipid Microarrays

Susan K Halstead  1 , Dawn Gourlay  1 , Hugh J Willison  2
Affiliations

Detection of Autoantibodies Using Combinatorial Glycolipid Microarrays

Susan K Halstead et al. Methods Mol Biol. 2022.

Abstract

Glycolipids cluster in plasma membranes to form heterogenous patches. Whereas lectins and antibodies have been conventionally viewed as binding a single oligosaccharide head group, and assayed accordingly, it is increasingly evident that cis-interactions between glycan headgroups may form unique molecular shapes that either enhance or attenuate binding of antibodies and other proteins. Herein we describe a method for assaying antibody binding to heteromeric glycolipid complexes that allows rapid, simple, inexpensive and high-throughput assessment of binding events, focusing on autoantibodies present in human serum.

Keywords: Autoimmune disease; Ganglioside; Glycoarray; Glycolipid; Guillain Barré syndrome; Immunoassay; Microarray.

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