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. 2022:2415:1-17.
doi: 10.1007/978-1-0716-1904-9_1.

Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

Debadrita Pal  1 , Florencia Visconti  1 , Silvia P Sepúlveda-Ramírez  1 , S Zachary Swartz  2 , Charles B Shuster  3
Affiliations

Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

Debadrita Pal et al. Methods Mol Biol. 2022.

Abstract

The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the absence of transgenic lines, alternative strategies must be employed to visualize microtubules and actin. Here, we describe methods to visualize actin and microtubule using either purified, recombinant proteins, or probes in in vitro-transcribed mRNAs.

Keywords: Confocal microscopy; Cytokinesis actin; Meiosis; Microtubule; Mitosis; Sea star; Sea urchin.

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