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. 2022 Jan 4;50(1):3.
doi: 10.1186/s41182-021-00386-0.

The 2017 Dengue virus 1 outbreak in northern Vietnam was caused by a locally circulating virus group

Taichiro Takemura  1 , Co Thach Nguyen  2   3   4 , Ha Chau Pham  1 , Thuy Trang Nguyen  1 , Vu Mai Phuong Hoang  5 , Le Khanh Hang Nguyen  5 , Takeshi Nabeshima  1 , Thi Thu Thuy Nguyen  5 , Thi Quynh Mai Le  5 , Meng Ling Moi  2   6 , Kouichi Morita  2 , Futoshi Hasebe  7
Affiliations

The 2017 Dengue virus 1 outbreak in northern Vietnam was caused by a locally circulating virus group

Taichiro Takemura et al. Trop Med Health. .

Abstract

Background: Dengue virus (DENV) is a member of insect vector-borne viruses, and it causes dengue fever. Southeast Asia is the epi-center of dengue fever in the world. The characterization of the virus is essential to identify the transmission and evolution of DENV.

Objectives: In 2017, there was an outbreak of Dengue virus type 1 (DENV1) in northern Vietnam and the neighboring countries. To identify the genetic character of the outbreak virus in the area, we conducted whole-genome sequencing analysis on the samples positive for the DENV1 along with real-time PCR.

Study design: In total, 1026 blood samples were collected from patients with suspected dengue fever in Ha Nam and Hai Duong province, nearby areas of the capital of Vietnam. After screening by real-time PCR, 40 of DENV1 positive samples were subjected to whole-genome sequencing, and 28 complete coding sequences were obtained.

Results: All 28 sequences were genotype I of DENV1, which is dominant in the southeast and East Asian countries. The phylogenetic analysis of the E region showed that they fell into a single cluster with the reported sequences from Vietnam between 2009 and 2016, in which the isolates from other countries are very rare. Our results suggested that the 2017 outbreak in the area was caused by locally circulating viruses.

Keywords: Dengue virus; Vietnam; Viral genome; Virus circulation.

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Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
a Phylogenetic tree constructed by the whole genome sequence together with DENV1-4 standard strains and genotype I, II, IV and V sequences. The sample lists are attached to the supplementary material. The accession numbers of newly defined samples are also described in supplementary materials. b Expanded phylogenetic tree constructed by the complete E gene sequence together with genotype I isolates recorded in the ViPR database from 6 southeast or East Asian countries. Open circle: the Vietnames isolates between 2006 and 2008; Open triangle: between 2009 and 2011; Open square: between 2012 and 2014: black triangle: between 2015 and 2016: black circle: 2017, analyzed in this study
See this image and copyright information in PMC

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