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. 2022:2407:91-96.
doi: 10.1007/978-1-0716-1871-4_8.

Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF)

Claudia Alteri  1   2 , Lorenzo Piermatteo  3 , Francesca Ceccherini Silberstein  3 , Valentina Svicher  3 , Carlo Federico Perno  4   5
Affiliations

Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF)

Claudia Alteri et al. Methods Mol Biol. 2022.

Abstract

Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the "hard-core" reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte-macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.

Keywords: Cell culture; GM-CSF; HIV; MDM; Monocytes/macrophages; Reservoir.

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