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. 2022:2407:115-154.
doi: 10.1007/978-1-0716-1871-4_11.

Hematopoietic Stem and Progenitor Cells (HSPCs)

Valeri H Terry  1 , Gretchen E Zimmerman  1 , Maria C Virgilio  2   3 , Mark M Painter  4 , Dale Bixby  1 , Kathleen L Collins  5   6
Affiliations

Hematopoietic Stem and Progenitor Cells (HSPCs)

Valeri H Terry et al. Methods Mol Biol. 2022.

Abstract

Cord blood is a readily available source of hematopoietic stem and progenitor cells (HSPCs) which can be infected with HIV-1 in vitro to produce inducible latently infected cells for reactivation studies. Infected HSPCs can also be found in the setting of clinically undetectable viremia in vivo. Here we describe an in vitro infection model utilizing cord blood derived HSPCs, as well as methods for isolating and characterizing provirus from bone marrow HSPCs from suppressed patients.

Keywords: HIV-1; HSPC; Latency; Reactivation; Reservoir.

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References

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