doi: 10.1007/978-1-0716-1871-4_15.
Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond
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- PMID: 34985667
- DOI: 10.1007/978-1-0716-1871-4_15
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Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond
Methods Mol Biol.
2022.
Abstract
The human decidua basalis, main uterine mucosa during pregnancy, provides an ex vivo model for studying natural protection of macrophages against HIV-1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.
Keywords: Decidua basalis; Decidual Macrophages (dM); Human Immunodeficiency Virus-1 (HIV-1); Infection; Pregnancy.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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References
-
- Jabrane-Ferrat N (2019) Features of human decidual NK cells in healthy pregnancy and during viral infection. Front Immunol 10:1397. https://doi.org/10.3389/fimmu.2019.01397 - DOI - PubMed - PMC
-
- Turco MY, Moffett A (2019) Development of the human placenta. Development 146(22):dev163428. https://doi.org/10.1242/dev.163428 - DOI - PubMed
-
- Backe E, Jimenez E, Unger M, Schafer A, Jauniaux E, Vogel M (1992) Demonstration of HIV-1 infected cells in human placenta by in situ hybridisation and immunostaining. J Clin Pathol 45(10):871–874. https://doi.org/10.1136/jcp.45.10.871 - DOI - PubMed - PMC
-
- Marlin R, Nugeyre MT, de Truchis C, Berkane N, Gervaise A, Barre-Sinoussi F, Menu E (2009) Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa. PLoS One 4(6):e5971. https://doi.org/10.1371/journal.pone.0005971 - DOI - PubMed - PMC
-
- El Costa H, Quillay H, Marlin R, Cannou C, Duriez M, Benjelloun F, de Truchis C, Rahmati M, Ighil J, Barre-Sinoussi F, Nugeyre MT, Menu E (2016) The local environment orchestrates mucosal decidual macrophage differentiation and substantially inhibits HIV-1 replication. Mucosal Immunol 9(3):634–646. https://doi.org/10.1038/mi.2015.87 - DOI - PubMed
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