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. 2022 Jan 6;17(1):e0262339.
doi: 10.1371/journal.pone.0262339. eCollection 2022.

Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis

Omayma O Abdelaleem  1 , Shereen Rashad Mohammed  1 , Hassan S El Sayed  1 , Sherin Khamis Hussein  2 , Doaa Y Ali  3 , Mostafa Y Abdelwahed  4 , Sylvana N Gaber  5 , Nada F Hemeda  6 , Rehab G Abd El-Hmid  2
Affiliations

Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis

Omayma O Abdelaleem et al. PLoS One. .

Abstract

Background: Neonatal sepsis is a serious condition. Recent clinical studies have indicated that microRNAs (miRNAs) are key players in the pathogenesis of sepsis, which could be used as biomarkers for this condition.

Patients and methods: A total of 90 neonates with sepsis and 90 healthy neonates were enrolled in this study. qRT-PCR was performed to measure the expression levels of serum miR-34a-5p and miR-199a-3p.

Results: miR-34a-5p and miR-199a-3p serum levels were significantly reduced in neonates with sepsis compared with those in healthy neonates (P = 0.006 and P = 0.001, respectively). Significant correlations of miR-34a-5p and miR-199a-3p with each of TLC, RDW, RBS, and C-reactive protein (CRP) as well as SNAPII were observed, indicating their associations with the severity of neonatal sepsis.

Conclusion: miR-34a-5p and miR-199a-3p may be useful as novel biomarkers in neonatal sepsis and may provide a new direction for its treatment.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Box plot representation demonstrating the serum expression of miR-34a-5p and miR-199a-3p (fold change) in neonatal sepsis compared with the levels in healthy neonates.
Data are presented as a box plot (median, upper, and lower quartiles). The horizontal line represents the expression levels of miR-34a-5p and miR-199a-3p in healthy neonates.
Fig 2
Fig 2. ROC curve analysis of serum miR-34a-5p and miR-199a-3p for differentiating neonates with sepsis from healthy ones.
See this image and copyright information in PMC

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