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. 2022 Mar;37(3):729-741.
doi: 10.1007/s11011-021-00871-9. Epub 2022 Jan 7.

Effect of Solanum vegetables on memory index, redox status, and expressions of critical neural genes in Drosophila melanogaster model of memory impairment

Opeyemi B Ogunsuyi  1   2   3 , Olawande C Olagoke  1 , Blessing A Afolabi  4 , Julia S Loreto  1 , Adedayo O Ademiluyi  2 , Michael Aschner  5 , Ganiyu Oboh  2 , Nilda V Barbosa  1 , João B T da Rocha  6
Affiliations

Effect of Solanum vegetables on memory index, redox status, and expressions of critical neural genes in Drosophila melanogaster model of memory impairment

Opeyemi B Ogunsuyi et al. Metab Brain Dis. 2022 Mar.

Abstract

African eggplant (Solanum macrocarpon L) (AE) and Black Nightshade (Solanum nigrum L) (BN) leaves are green leafy vegetables with nutritional and ethnobotanical values. We have previously characterized the vegetables via HPLC/LC-MS to reveal notable phenolic acids, flavonoids and alkaloids. In this present study, we addressed the efficacy of the two vegetables in mitigating mercuric chloride (HgCl2)-induced neurotoxicity and memory impairment in Drosophila melanogaster. Flies were exposed to HgCl2 (0.30 mg/g) alone or in combination with the vegetables (0.1 and 1.0%) of both samples in their diets for seven days. The results showed that HgCl2 (Hg)-exposed flies had significantly reduced survival rate and memory index, which were ameliorated in the Hg-exposed flies fed AE or BN. This was accompanied by increased reactive oxygen species (ROS) levels, reduced total thiol, as well as catalase, glutathione transferase (GST) and acetylcholine esterase (AChE) activities in Hg-exposed fly heads, but ameliorated in Hg-exposed flies fed dietary inclusions of the vegetables. In addition, the Hg-induced alterations in SOD, NF-ҝB/Relish, Dronc and Reaper mRNA levels were statistically indistinguishable from controls in Hg-treated flies fed diets containing AE or BN. Normalization of cnc/Nrf2 and FOXO were observed only in Hg-treated flies fed BN. These findings suggest that dietary AE or BN leaves offer protection against Hg-induced memory impairment and neurotoxicity in D. melanogaster, and further justify them as functional foods with neuroprotective properties.

Keywords: Antioxidant; Functional foods; Memory; Neurotoxicity; Solanum spp..

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References

    1. Abdel Moneim AE (2015) The neuroprotective effect of berberine in mercury-induced neurotoxicity in rats. Metab Brain Dis. https://doi.org/10.1007/s11011-015-9652-6
    1. Abolaji A, Kamdem JP, Farombi O, et al (2013) Drosophila melanogaster as a Promising Model Organism in Toxicological Studies: A Mini Review Ameliorative Properties of Citrus Bioflavonoids on 4-vinylcyclohexene diepoxide (VCD)-induced reprotoxicity View project Nigerian Drosophila Research Community V. Arch Basic Appl Med 1 33 - 38 1:33–38
    1. Adedara IA, Rosemberg DB, Souza DO et al (2015) Biochemical and behavioral deficits in the lobster cockroach Nauphoeta cinerea model of methylmercury exposure. Toxicol Res (Camb). https://doi.org/10.1039/c4tx00231h
    1. Adedara IA, Abolaji AO, Rocha JBT, Farombi EO (2016) Diphenyl Diselenide protects against mortality, locomotor deficits and oxidative stress in Drosophila melanogaster model of manganese-induced neurotoxicity. Neurochem Res https://doi.org/10.1007/s11064-016-1852-x
    1. Aebi H (1984) [13] Catalase in Vitro. Methods Enzymol. https://doi.org/10.1016/S0076-6879(84)05016-3

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