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. 2022 Jan 5;7(1):391-393.
doi: 10.1093/jalm/jfab133.

Utility of Live Cell-Based Assays for Autoimmune Neurology Diagnostics

Mark Woodhall  1 , Victor Mgbachi  1 , Hannah Fox  1 , Sarosh Irani  1 , Patrick Waters  1
Affiliations

Utility of Live Cell-Based Assays for Autoimmune Neurology Diagnostics

Mark Woodhall et al. J Appl Lab Med. .
No abstract available

Keywords: AMPAR; AQP4; GABABR; LGI1; NMDAR; cell-based assay.

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Figures

Fig. 1
Fig. 1
(A), Live CBA showing a serum sample positive for aquaporin-4 antibodies (AQP4-Abs; red, 1) and negative on myelin oligodendrocyte glycoprotein (MOG)-expressing cells (2). A second serum sample is negative for AQP4-Abs (3) but positive for MOG-Abs (4 in green). Cell nuclei are labelled with dapi (blue); (B), Contactin-associated, protein-like 2 (CASPR2) antibody-positive serum shows a distinct hippocampal staining pattern on rodent central nervous system tissue and on primary neuronal cultures (patient IgG binding shown in green; neurons stained with antibodies to MAP2 (microtubule-associated protein 2) in red). This contrasts with healthy control serum which is negative on IHC and primary cultures; (C), Data from 3 studies demonstrate significant numbers of false-negative serum and cerebrospinal fluid (CSF) test results on commercial CBAs for multiple antibody targets in autoimmune neurology. Missed cases in (C) were positive by IHC, research CBAs, and supported by clinical data where available.
See this image and copyright information in PMC

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