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. 2021 Dec 29:14:10423-10428.
doi: 10.2147/IJGM.S343889. eCollection 2021.

Correlation Analysis of circRNA Circ_0071662 in Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma

Lei Xia  1 , Wei Liang  2 , Dan Que  2 , Qichao Xie  2
Affiliations

Correlation Analysis of circRNA Circ_0071662 in Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma

Lei Xia et al. Int J Gen Med. .

Abstract

Background: The role of circRNA circ_0071662 has been studied in bladder cancer. The present study aimed to analyze its involvement in esophageal squamous cell carcinoma (ESCC).

Methods: Patients with ESCC (n = 66), esophageal ulcer (EU, n = 66), or gastroesophageal reflux disease (GERD, n = 66) and healthy controls (n = 66) were enrolled in this study. Plasma samples were collected from all patients and controls. ESCC and paired non-tumor tissue samples were collected from ESCC patients. Circ_0071662 levels in these samples were determined by RT-qPCRs. Diagnostic and prognostic values of circ_0071662 for ESCC were analyzed with ROC curve and survival curve analyses.

Results: Circ_0071662 level was decreased in ESCC, but not in GERN and EU compared to the controls and in ESCC tissues compared to the non-tumor tissues. Plasma circ_0071662 was closely correlated with patients' tumor size but not with other clinical features. Decreased plasma circ_0071662 levels separated ESCC patients from GERN patients, EU patients, and healthy controls. Low plasma circ_0071662 levels were closely correlated with worse survival outcomes of ESCC patients.

Conclusion: Circ_0071662 is lowly expressed in ESCC and may serve as a potential diagnostic and prognostic biomarker for ESCC.

Keywords: circ_0071662; diagnosis; esophageal squamous cell carcinoma; prognosis.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
RT-qPCR analysis of t circ_0071662 levels in tissues and plasma. RNA samples were isolated from paired ESCC and non-tumor tissue samples from 66 ESCC patients (A) and plasma samples from ESCC patients (n = 66), EU patients (n = 66), GERD patients (n = 66), and controls (n = 66) (B), and subjected to cDNA synthesis and subsequent qPCR to determine the expression of circ_0071662Circ_0071662 levels. **p < 0.01.
Figure 2
Figure 2
Diagnostic values of plasma circulating circ_0071662 for ESCC. ROC curve analysis was carried out with ESCC patients as the true positive cases and EU (A), GERD (B), or controls (C) as the true negative cases to analyze the role of plasma circulating circ_0071662 in the diagnosis of ESCC.
Figure 3
Figure 3
Analysis of the role of plasma circulating circ_0071662 in predicting the survival outcomes of ESCC patients. The 66 ESCC patients were grouped into high and low plasma circ_0071662 level groups. The survival curves of both circ_0071662 level groups were plotted using the 5-year survival data and compared using the Log rank test.
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