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. 2021 Nov 22:18:94.
doi: 10.4103/1735-3327.330875. eCollection 2021.

Drug release kinetics and biological properties of a novel local drug carrier system

Farhad Shafiei  1   2 , Mehrsima Ghavami-Lahiji  3 , Tahereh Sadat Jafarzadeh Kashi  1   2 , Farhood Najafi  4
Affiliations

Drug release kinetics and biological properties of a novel local drug carrier system

Farhad Shafiei et al. Dent Res J (Isfahan). .

Abstract

Background: The purpose of this in vitro study was to investigate drug release kinetics and cytotoxicity of a novel drug delivery system for treatment of periodontitis.

Materials and methods: This in vitro study addresses the fabrication of a polycaprolactone/alginic acid-based polymeric film loaded with metronidazole, as a basic drug in the treatment of periodontal diseases. Films were prepared by solvent casting technique. Four formulations with different percentages of drug by weight (3%, 5%, 9%, and 13%) were prepared. Drug release kinetics were investigated using ultraviolet-visible spectroscopy during (one week). Data were analyzed using repeated measures ANOVA. Cytotoxicity of drug-loaded system extracts was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 24-h incubation. The results were evaluated according to ISO standard 10993-5 and assessed using ANOVA and Tukey's tests at a significance level of P < 0.05.

Results: All polymeric films showed a burst drug release followed by a gradual release. Drug release data were fitted well with the first-order kinetic model in all drug-containing formulations indicating that drug release is a fraction of remaining drug in the matrix. Drug release is mainly driven by diffusion of medium into the composite matrix. 3%wt metronidazole-containing formulation exhibited the best MTT result.

Conclusion: The findings of this study supported the synthesis of drug-loaded periodontal films with 3% metronidazole due to better biological properties along with the ability of acceptable drug release to eradicate anaerobic periodontal bacteria.

Keywords: Drug delivery system; metronidazole; periodontal diseases; pharmacokinetics; toxicity.

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Conflict of interest statement

The authors of this manuscript declare that they have no conflicts of interest, real or perceived, financial or nonfinancial in this article.

Figures

Figure 1
Figure 1
Calibration curve of metronidazole drug in phosphate-buffered saline.
Figure 2
Figure 2
In vitro release of metronidazole during 1 week.
Figure 3
Figure 3
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results of different dilutions of the polymeric film extract. (CM: Culture medium), (M1, 3%; M2, 5%; M3, 9%; and M4, 13% metronidazole-loaded polymeric film).
Figure 4
Figure 4
The scanning electron microscopy micrographs of the cell morphology on the surface of polymeric films at ×1000 (a) and ×2000 (b) magnifications.
See this image and copyright information in PMC

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