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. 2021 Dec 31:2021:6568477.
doi: 10.1155/2021/6568477. eCollection 2021.

DCI after Aneurysmal Subarachnoid Hemorrhage Is Related to the Expression of MFG-E8

Xianjun Chen  1 , Yong'an Jiang  1 , Jiayu Liu  2 , Changfeng Wang  1 , Dengfeng Wan  1 , Ai'jun Liang  1 , Jingxing Leng  1 , Yu Yang  1 , Hui Xiang  1 , Ru'en Liu  1   2
Affiliations

DCI after Aneurysmal Subarachnoid Hemorrhage Is Related to the Expression of MFG-E8

Xianjun Chen et al. Biomed Res Int. .

Abstract

Objective: To explore the predictive value of milk fat globule epidermal growth factor 8 (MFG-E8) in the occurrence of delayed cerebral ischemia (DCI) after an aneurysmal subarachnoid hemorrhage (aSAH).

Methods: We recruited 32 patients with aSAH as the case group and 24 patients with unruptured aneurysms as the control group. Serum MFG-E8 levels were measured by western blot and enzyme-linked immunosorbent assay. We analyzed the relationship between MFG-E8 levels and the risk of DCI.

Results: The levels of serum MFG-E8 in the case group (mean = 11160.9 pg/mL) were significantly higher than those in the control group (mean = 3081.0 pg/mL, p < 0.001). MFG-E8 levels highly correlated with the World Federation of Neurosurgical Societies (WFNS) and modified Fisher scores (r = -0.691 and - 0.767, respectively, p < 0.001). In addition, MFG-E8 levels in patients with DCI (5882.7 ± 3162.4 pg/mL) were notably higher than those in patients without DCI (15818.2 ± 3771.6 pg/mL, p < 0.001). A receiver operating characteristic curve showed that the occurrence of DCI could effectively be predicted by MFG-E8 (area under the curve = 0.976, 95%CI = 0.850-1.000). Kaplan-Meier survival analysis showed a remarkable decrease in the incidence of DCI in case group individuals with high levels of MFG-E8 (≥11160.9 pg/mL, p < 0.001).

Conclusion: MFG-E8 may be a useful predictive marker for DCI after an aSAH and could be a promising surrogate end point.

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Conflict of interest statement

The authors declare that there is no conflict of interest regarding the publication of this article.

Figures

Figure 1
Figure 1
MFG-E8 levels in the aSAH and the non-aSAH groups. (a) Three patients were randomly selected from the non-aSAH group (N1~N3), and five patients were randomly selected from the aSAH group (C1~C5). The expression of MFG-E8 in the aSAH group was significantly higher than that in the control group. (b) ELISA was performed on the serum samples of all patients in both groups, and the results were consistent with those obtained using western blotting; the expression of MFG-E8 increased significantly in the case group.
Figure 2
Figure 2
Flowchart of enrolled subjects.
Figure 3
Figure 3
MFG-E8 levels in the DCI and non-DCI groups.
Figure 4
Figure 4
Relationship between biomarkers and clinical scores in patients with aSAH. Relationship between (a) WFNS scores and MFG-E8, (b) modified Fisher scores and MFG-E8, and (c) WFNS and modified Fisher scores.
Figure 5
Figure 5
Predictive significance of modified Fisher scores, MFG-E8 level, and WFNS scores in DCI.
Figure 6
Figure 6
Kaplan–Meier curve of survival rates in patients with DCI.
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