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. 2022 Jan 10;17(1):e0262484.
doi: 10.1371/journal.pone.0262484. eCollection 2022.

Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome

Klara Pecankova  1 , Pavla Pecherkova  1 , Zdenka Gasova  1 , Zofie Sovova  1 , Tomas Riedel  2 , Eliézer Jäger  2 , Jaroslav Cermak  1 , Pavel Majek  1
Affiliations

Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome

Klara Pecankova et al. PLoS One. .

Abstract

Background: Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hematological malignancies.

Objective: Here, we investigated proteome changes in the exosome-rich fraction of the plasma of myelodysplastic syndrome patients and healthy donors.

Methods: Exosome-rich fraction of the plasma was isolated using ExoQuick™: proteomes were compared and statistically processed; proteins were identified by nanoLC-MS/MS and verified using the ExoCarta and QuickGO databases. Mann-Whitney and Spearman analyses were used to statistically analyze the data. 2D western blot was used to monitor clusterin proteoforms.

Results: Statistical analyses of the data highlighted clusterin alterations as the most significant. 2D western blot showed that the clusterin changes were caused by posttranslational modifications. Moreover, there was a notable increase in the clusterin proteoform in the exosome-rich fraction of plasma of patients with more severe myelodysplastic syndrome; this corresponded with a simultaneous decrease in their plasma.

Conclusions: This specific clusterin proteoform seems to be a promising biomarker for myelodysplastic syndrome progression.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Characterization of the isolated ERF by DLS.
DLS data interpretation was performed considering the parameter of intensity.
Fig 2
Fig 2. Positions of ERF proteome spots.
Positions of ERF proteome spots significantly differing in normalized volumes (circles with numbers) and positions of spots selected on the basis of the Spearman correlation coefficient test (spots with normalized volumes correlating strongly in the MDS groups are highlighted in green; spots with normalized volumes correlating strongly in the control group are highlighted in orange). Brightness and contrast of the gel image were adjusted for clearer illustration.
Fig 3
Fig 3. Two-dimensional western blot analysis of clusterin.
Panel A represents an illustrative clusterin spot pattern. The arrow indicates the specific clusterin spot (A1–A5) with its expression profiles in plasma (B1–B5) and exosome-rich fraction (C1–C5). Fold values for each spot (A1–A5) are presented for plasma (B6) and exosome-rich fraction (C6).
See this image and copyright information in PMC

References

    1. Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A, et al.. The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: Rationale and important changes. Blood. 2009. pp. 937–951. doi: 10.1182/blood-2009-03-209262 - DOI - PubMed
    1. Brunning RD, Bennett JM, Flandrin G, Matutes E, Head D, Vardiman JW, et al.. Myelodysplastic syndromes and acute myeloid leukemias. 1st ed. In: Jaffe ES, Harris NL, Stein H, Vardiman JW, editors. World Health Organization Classification of Tumours Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues. 1st ed. Lyon: IARC Press; 2001. pp. 61–106.
    1. Robbins PD, Morelli AE. Regulation of immune responses by extracellular vesicles. Nat Rev Immunol. 2014;14: 195–208. doi: 10.1038/nri3622 - DOI - PMC - PubMed
    1. Utleg AG, Yi EC, Xie T, Shannon P, White JT, Goodlett DR, et al.. Proteomic analysis of human prostasomes. Prostate. 2003;56: 150–161. doi: 10.1002/pros.10255 - DOI - PubMed
    1. Pisitkun T, Shen R-F, Knepper MA. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004;101: 13368–13373. doi: 10.1073/pnas.0403453101 - DOI - PMC - PubMed

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