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. 2022 Mar;101(3):101635.
doi: 10.1016/j.psj.2021.101635. Epub 2021 Dec 9.

Enhancement of sperm quality and fertility-related parameters in Hubbard grandparent rooster fed diets supplemented with soybean lecithin and vitamin E

Shahram Shabani  1 , Morteza Mehri  2 , Fatemeh Shirmohammad  1 , Mohsen Sharafi  3
Affiliations

Enhancement of sperm quality and fertility-related parameters in Hubbard grandparent rooster fed diets supplemented with soybean lecithin and vitamin E

Shahram Shabani et al. Poult Sci. 2022 Mar.

Abstract

The purpose of this study was to investigate the effect of dietary supplementation of different levels of soybean lecithin and vitamin E on semen quality parameters and some reproductive hormones in Hubbard grandparent roosters. The experiment was conducted in a 3 × 2 factorial arrangement with 3 levels of soybean lecithin (0, 1, and 2%) and 2 levels of vitamin E (0 and 300 mg/kg). Semen samples were collected on d 0, 20, 40 and 60 of the experiment and analyzed. Adding 1% soybean lecithin and vitamin E into the diet increased semen volume and sperm concentration, membrane integrity and viability (P < 0.05). Supplementing diets with 1 or 2% lecithin in addition to vitamin E significantly improved total motility and progressive motility (P < 0.05). Vitamin E significantly increased the amplitude of lateral head displacement (ALH) of sperm (P < 0.05). Although there was no effect on LH and FSH when diets were supplemented with vitamin E and 1 or 2% lecithin, testosterone concentration was increased (P < 0.05). Malondialdehyde (MDA) concentration was significantly lower in all 3 treatments containing vitamin E (P < 0.05). It can be concluded that supplementation of rooster diets with vitamin E and 1% lecithin can improve fertility related parameters in Hubbard grandparent roosters.

Keywords: soybean lecithin; sperm motility; vitamin E.

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Figures

Figure 1
Figure 1
Effect of vitamin E × time interaction on total sperm motility (A) and on progressive motility (B); and lecithin × time interaction on total sperm motility (C) and on STR (D) in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60 d. Total sperm motility, progressive motility and STR were measured by sperm class analysis software (SCA). Superscripts on different means within each day differ significantly at P ≤ 0.05.
Figure 2
Figure 2
Effect of lecithin × time interaction (A) and vitamin × time interaction (B) on plasma testosterone in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60 d. Testosterone was measured by chicken ELISA kit. Superscripts on different means within each day differ significantly at P ≤ 0.05.
Figure 3
Figure 3
Effect of lecithin × time interaction (A) and vitamin × time interaction (B) on sperm concentration in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60 d. Sperm concentration was measured by Neubauer hemocytometer. Superscripts on different means within each day differ significantly at P ≤ 0.05.
Figure 4
Figure 4
Effect of lecithin × time interaction on membrane integrity in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60d. Membrane integrity was measured by hypo osmotic swelling test (HOST). Superscripts on different means within each day differ significantly at P ≤ 0.05.
Figure 5
Figure 5
Effect of vitamin E × time interaction (A) and vitamin × time interaction (B) on viability in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60 d. Viability was measured by Annexin V-FITC kit. Superscripts on different means within each day differ significantly at P ≤ 0.05.
Figure 6
Figure 6
Effect of vitamin E × time interaction on seminal MDA in 45-wk-old Hubbard M99 Line A grandparent roosters fed diets for 60 d. MDA was measured by of thiobarbituric acid. Superscripts on different means within each day differ significantly at P ≤ 0.05.
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