Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr 26;6(8):2695-2699.
doi: 10.1182/bloodadvances.2021006232.

CRISPR/Cas9 genome-edited universal CAR T cells in patients with relapsed/refractory lymphoma

Yelei Guo  1 , Chuan Tong  1 , Liping Su  2 , Wenying Zhang  1 , Hejin Jia  1 , Yang Liu  1 , Qingming Yang  1 , Zhiqiang Wu  1 , Yao Wang  1 , Weidong Han  1   3
Affiliations

CRISPR/Cas9 genome-edited universal CAR T cells in patients with relapsed/refractory lymphoma

Yelei Guo et al. Blood Adv. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Toxicities, persistence, and response in patient 1 after administration of universal CAR T cells. (A) The change in maximum temperature (Tmax) and the serum level of C-reactive protein (CRP) were tested in patient 1 before and after universal CAR (UCAR) T-cell infusion and CRP recovery after multicycle of methylprednisolone (red arrow), tocilizumab (light green arrow), and etanercept (purple arrow) administration. (B) The serum levels of cytokines, including interleukin-2 (IL-2), IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α), were tested after UCAR T-cell infusion. (C) Chest X-ray showed the appearance of acute pulmonary edema after UCAR T-cell infusion. (D) Persistence of infused UCAR T cells in patient peripheral blood (PB) before and after cell infusion. The level of UCAR T cells was analyzed using quantitative polymerase chain reaction to detect the CAR gene copy number in genomic DNA obtained from PB of patient 1. (E) The change in B-cell number before and after UCAR T-cell infusion. (F) Skin damage in the right trunk developed after UCAR T-cell infusion. The swelling improved after methylprednisolone treatment.
Figure 2.
Figure 2.
Toxicities, persistence, and response in patient 2 after administration of universal CAR T cells. (A) The change in maximum temperature (Tmax) and the serum level of C-reactive protein (CRP) were monitored in patient 2 before and after universal CAR (UCAR) T-cell infusion, and Tmax and CRP recovered without any treatment. (B) The serum levels of cytokines, including interleukin-2 (IL-2), IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α), were tested before and after UCAR T-cell infusion. (C) Persistence of the infused UCAR T cells in peripheral blood (PB) of patient 2 before and after cell infusion. Flow cytometry and quantitative polymerase chain reaction were used to detect the level of UCAR T cells in PB. (D) The change in B-cell number before and after UCAR T-cell infusion. (E) The change in NK cell number before and after UCAR T-cell infusion.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Tong C, Zhang Y, Liu Y, et al. . Optimized tandem CD19/CD20 CAR-engineered T cells in refractory/relapsed B-cell lymphoma. Blood. 2020;136(14):1632-1644. - PMC - PubMed
    1. Schuster SJ, Bishop MR, Tam CS, et al. ; JULIET Investigators . Tisagenlecleucel in adult relapsed or refractory diffuse large B-cell lymphoma. N Engl J Med. 2019;380(1):45-56. - PubMed
    1. Porter DL, Levine BL, Kalos M, Bagg A, June CH. Chimeric antigen receptor-modified T cells in chronic lymphoid leukemia. N Engl J Med. 2011;365(8):725-733. - PMC - PubMed
    1. Kebriaei P, Singh H, Huls MH, et al. . Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells. J Clin Invest. 2016;126(9):3363-3376. - PMC - PubMed
    1. MacLeod DT, Antony J, Martin AJ, et al. . Integration of a CD19 CAR into the TCR alpha chain locus streamlines production of allogeneic gene-edited CAR T cells. Mol Ther. 2017;25(4):949-961. - PMC - PubMed

Publication types