Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan 5;11(1):139.
doi: 10.3390/plants11010139.

An R2R3-MYB Transcription Factor OsMYBAS1 Promotes Seed Germination under Different Sowing Depths in Transgenic Rice

Xiaomin Wang  1 , Rong Wu  1 , Tongshu Shen  1 , Zhenan Li  1 , Chengyong Li  2 , Bangkui Wu  1 , Hongye Jiang  1 , Guangwu Zhao  1
Affiliations

An R2R3-MYB Transcription Factor OsMYBAS1 Promotes Seed Germination under Different Sowing Depths in Transgenic Rice

Xiaomin Wang et al. Plants (Basel). .

Abstract

MYB-type transcription factors play essential regulatory roles in seed germination and the response to seedling establishment stress. This study isolated a rice R2R3-MYB gene, OsMYBAS1, and functionally characterized its role in seed germination by generating transgenic rice plants with the overexpression and knockout of OsMYBAS1. Gene expression analysis suggested that OsMYBAS1 was highly expressed in brown rice and root, respectively. Subcellular localization analysis determined that OsMYBAS1 was localized in the nucleus. No significant differences in seed germination rate were observed among wild-type (WT) and transgenic rice plants at the 0-cm sowing depth. However, when sown at a depth of 4 cm, higher germination rates, root lengths and seedling heights were obtained in OsMYBAS1-overexpressing plants than in WT. Furthermore, the opposite results were recorded between the osmybas1 mutants and WT. Moreover, OsMYBAS1-overexpressing plants significantly enhanced superoxide dismutase (SOD) enzyme activity and suppressed the accumulation of malondialdehyde (MDA) content at the 4-cm sowing depth. These results indicate that the MYB transcription factor OsMYBAS1 may promote rice seed germination and subsequent seedling establishment under deep-sowing conditions. These findings can provide valuable insight into rice seed-quality breeding to facilitate the development of a dry, direct-seeding production system.

Keywords: MYB transcription factor; OsMYBAS1; antioxidant enzyme; deep-sowing; seed germination.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
qRT-PCR analysis of the expression of OsMYBAS1 in different rice tissues. Data are mean ± SE of three biological replications. Asterisks indicate statistically significant differences (p < 0.05, Duncan test) from the control (glume). Actin was used as an internal control.
Figure 2
Figure 2
Subcellular localization analysis of OsMYBAS1. Confocal images of rice protoplasts cells under the GFP channel showing the constitutive localization of GFP and the nuclear localization of OsMYBAS1-GFP. Confocal images of rice protoplasts cells under the mCherry channel showing the constitutive localization of mCherry. The merged images of GFP and OsMYBAS1-GFP are presented, respectively.
Figure 3
Figure 3
Identification and phenotype of OsMYBAS1-overexpressing plants and osmybas1 mutants. (A) Relative expression of wild-type (WT) and two OsMYBAS1-overexpressing rice lines (OE-1 and OE-2); (B) leaf phenotype of WT and osmybas1 mutants (osmybas1-1 and osmybas1-2) soaked in Hygromycin solution; (C) two osmybas1 mutants (osmybas1-1 and osmybas1-2) were obtained by sequencing and the knockout sites are presented; (D) phenotype of WT, OsMYBAS1-overexpressing plants and osmybas1 mutants at the sowing depths of 0 and 4 cm, respectively. Data are mean ± SE of three biological replications. Asterisks indicate statistically significant differences (p < 0.05, Duncan test) from the control (glume). Actin was used as an internal control.
See this image and copyright information in PMC

References

    1. Seck P.A., Diagne A., Mohanty S., Wopereis M.C.S. Crops that feed the world 7: Rice. Food Secur. 2012;4:7–24. doi: 10.1007/s12571-012-0168-1. - DOI
    1. Yang W.J., Xu D.T.S., Li S.Y., Tang X.G., Pan S.G., Chen X.F., Mo Z.W. Emergence and seedling establishment of rice varieties at different sowing depths. J. Plant Growth Regul. 2021 doi: 10.1007/s00344-021-10408-0. - DOI
    1. Wang W.Q., Peng S.B., Chen Q., Mei J.H., Dong H.L., Nie L.X. Effects of pre-sowing seed treatments on establishment of dry direct-seeded early rice under chilling stress. AoB Plants. 2016;8:plw074. doi: 10.1093/aobpla/plw074. - DOI - PMC - PubMed
    1. Liu H.Y., Hussain S., Zheng M.M., Peng S.B., Huang J.J., Cui K.H., Nie L.X. Dry direct-seeded rice as an alternative to transplanted-flooded rice in Central China. Agron. Sustain. Dev. 2014;35:285–294. doi: 10.1007/s13593-014-0239-0. - DOI
    1. Wang W.X., Du J., Zhou Y.Z., Zeng Y.J., Tan X.M., Pan X.H., Shi Q.H., Wu Z.M., Zeng Y.H. Effects of different mechanical direct seeding methods on grain yield and lodging resistance of early Indica rice in South China. J. Integr. Agr. 2021;20:1204–1215. doi: 10.1016/S2095-3119(20)63191-4. - DOI

LinkOut - more resources