Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec 31;11(1):99.
doi: 10.3390/foods11010099.

Lab Scale Extracted Conditions of Polyphenols from Thinned Peach Fruit Have Antioxidant, Hypoglycemic, and Hypolipidemic Properties

Kun Dai  1 , Yingying Wei  1 , Shu Jiang  1 , Feng Xu  1 , Hongfei Wang  1 , Xin Zhang  1 , Xingfeng Shao  1
Affiliations

Lab Scale Extracted Conditions of Polyphenols from Thinned Peach Fruit Have Antioxidant, Hypoglycemic, and Hypolipidemic Properties

Kun Dai et al. Foods. .

Abstract

Thinned peach polyphenols (TPPs) were extracted by ultrasonic disruption and purified using macroporous resin. Optimized extraction conditions resulted in a TPPs yield of 1.59 ± 0.02 mg GAE/g FW, and optimized purification conditions resulted in a purity of 43.86% with NKA-9 resin. TPPs composition was analyzed by UPLC-ESI-QTOF-MS/MS; chlorogenic acid, catechin, and neochlorogenic acid were the most abundant compounds in thinned peaches. Purified TPPs exhibited scavenging activity on DPPH, ABTS, hydroxyl radical, and FRAP. TPPs inhibited α-amylase and α-glucosidase by competitive and noncompetitive reversible inhibition, respectively. TPPs also exhibited a higher binding capacity for bile acids than cholestyramine. In summary, TPPs from thinned peaches are potentially valuable because of their high antioxidant, hypoglycemic, and hypolipidemic capacities, and present a new incentive for the comprehensive utilization of thinned peach fruit.

Keywords: UPLC-ESI-QTOF-MS/MS; antioxidation; hypoglycemic; hypolipidemic; polyphenols; thinned peach.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Effect of different extraction parameters on yield of polyphenols from thinned peach. Ultrasonic time (A), ultrasonic temperature (B), ultrasonic power (C), and solid-to-liquid ratio (D). Different letters indicate that there are significant differences (p < 0.05).
Figure 2
Figure 2
Response surface 3D plots showing combined effects of extraction parameters on yield of polyphenols from thinned peach. (A) Ultrasonic temperature and ultrasonic time; (B) ultrasonic power and ultrasonic temperature; (C) ultrasonic power and ultrasonic time; (D) solid-to-liquid ratio and ultrasonic temperature; (E) solid-to-liquid ratio and ultrasonic time; (F) solid-to-liquid ratio and ultrasonic power.
Figure 3
Figure 3
Absorption (A) and desorption (B) capacity of different resins; static adsorption (C) and desorption (D) kinetic curves of NKA-9. Different letters indicate that there are significant differences (p < 0.05).
Figure 4
Figure 4
Effects of different factors on adsorption and desorption: pH (A), sample concentration (B), adsorption speed (C), ethanol concentration (D), and desorption speed (E). Dynamic adsorption and desorption curves under optimal conditions (F). Different letters indicate that there are significant differences (p < 0.05).
Figure 5
Figure 5
UPLC-ESI-Q-TOF-MS/MS chromatograms of TPPs: (1) Neochlorogenic acid; (2) Cyanidin-3-glucoside; (3) Procyanidin B1; (4) Chlorogenic acid; (5) Catechin; (6) B-type (epi)catechin trimer; (7) Epicatechin; (8) Coumaroylquinic acid; (9) procyanidin C1; (10) Rutin; (11) Hyperoside; (12) Quercitrin; (13) Isorhamnetin-3-O-glucoside; (14) Quercetin.
Figure 6
Figure 6
Antioxidant capacity of TPPs: DPPH (A), ABTS (B), Hydroxyl radical (C), FRAP (D). * Indicates difference is significant (p < 0.05), ** indicates extremely significant (p < 0.01).
Figure 7
Figure 7
Inhibitory effects (A,B), inhibition kinetic curve (C,D) and inhibition modes (E,F) of TPPs against α-amylase and α-glucosidase. ** indicates extremely significant (p < 0.01).
Figure 8
Figure 8
Bile acid binding capacity of TPPs. (A) Sodium taurocholate binding capacity, (B) sodium glycocholate binding capacity. ** Indicates extremely significant (p < 0.01).
See this image and copyright information in PMC

References

    1. Xu C.P., Nasrollahzadeh M., Selva M., Issaabadi Z., Luque R. Waste-to-wealth: Biowaste valorization into valuable bio(nano)materials. Chem. Soc. Rev. 2019;48:4791–4822. doi: 10.1039/C8CS00543E. - DOI - PubMed
    1. Wei M., Wang H., Ma T., Ge Q., Fang Y., Sun X. Comprehensive utilization of thinned unripe fruits from horticultural crops. Foods. 2021;10:2043. doi: 10.3390/foods10092043. - DOI - PMC - PubMed
    1. Guo C.T., Bi J.F., Li X., Lyu J., Wu X.Y., Xu Y. Polyphenol metabolic diversity of Chinese peach and nectarine at thinned and ripe stages by UPLC-ESI-Q-TOF-MS combined with multivariate statistical analysis. J. Food Compos. Anal. 2020;90:103502. doi: 10.1016/j.jfca.2020.103502. - DOI
    1. Nasser M., Cheikh H., Hijazi A., Merah O., Rekaby A., Awada R. Phytochemical profile, antioxidant and antitumor activities of green grape juice. Processes. 2020;8:507. doi: 10.3390/pr8050507. - DOI
    1. Zheng H.Z., Kim Y.I., Chung S.K. A profile of physicochemical and antioxidant changes during fruit growth for the utilisation of unripe apples. Food Chem. 2012;131:106–110. doi: 10.1016/j.foodchem.2011.08.038. - DOI

LinkOut - more resources