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. 2022 Sep 1;53(5):e109-e112.
doi: 10.1093/labmed/lmab116.

A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood

Narin Khongjaroensakun  1 , Karan Paisooksantivatana  1 , Suttikarn Santiwatana  2   3 , Tulyapruek Tawonsawatruk  4 , Kantarat Kusolthammarat  1   5 , Praguywan Kadegasem  2 , Noppawan Tangbubpha  2 , Juthamard Chantaraamporn  2 , Ampaiwan Chuansumrit  2
Affiliations

A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood

Narin Khongjaroensakun et al. Lab Med. .

Erratum in

Abstract

Objectives: To determine and compare the platelet growth factors in human platelet lysate (HPL) prepared from citrated whole blood, with final centrifugations at 4oC and 25oC.

Methods: We collected specimens of citrated whole blood from 27 healthy volunteers. The platelet-rich plasma (PRP) was separated to prepare the HPL, which was further divided into 2 portions for the final centrifugation, at 4oC and 25oC, respectively. Platelet growth factors were measured and compared between the 2 groups.

Results: All platelet growth factors were higher than those in PRP prepared from citrated whole blood. Moreover, the final centrifugation at 25oC resulted in noninferiority of platelet-growth-factor level.

Conclusion: This study provided a simple method for small-volume of HPL preparation using only 10-15 mL of citrated whole blood. Further, the entire process of centrifugation can be performed at room temperature of 25oC, which is more applicable than lower temperatures for other laboratories.

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