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. 2022 Feb;19(2):293-295.
doi: 10.1038/s41423-021-00836-z. Epub 2022 Jan 11.

Increased immune escape of the new SARS-CoV-2 variant of concern Omicron

Jie Hu #  1 , Pai Peng #  1 , Xiaoxia Cao #  1 , Kang Wu  1 , Juan Chen  1 , Kai Wang  1 , Ni Tang  1 , Ai-Long Huang  2
Affiliations

Increased immune escape of the new SARS-CoV-2 variant of concern Omicron

Jie Hu et al. Cell Mol Immunol. 2022 Feb.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The effect of the Omicron variant on viral infectivity and immune escape. a Mutational landscapes in variants Omicron and D614G. b HEK293T-hACE2 cells or A549-hACE2 cells were infected with pseudotyped Omicron and D614G particles bearing all S mutations to determine viral infectivity using a luciferase assay. c HEK293T cells were transfected with the spike-expressing plasmids of Omicron and D614G variants and the immunoblots were probed with the indicated antibodies; the cleavage quantified by densitometry using ImageJ. d, e Pseudovirus-based neutralizing assays were performed to detect neutralizing activity of the sera from the convalescents (n = 12, sampled at around 30 days after symptom onset) (d) and vaccinated individuals with RBD subunit vaccine ZF2001 (n = 12, sampled at 15-60 days post the 3rd dose) (e) against SARS-CoV-2 Omicron and D614G variants. The half-maximal inhibitory dose (ID50) was indicated by geometric mean titers (GMT). The threshold of ID50 detection was 1:40. Statistical data analysis was performed using GraphPad Prism version 8.0 software. Student’s t-tests were used to compare between Omicron and D614G variants. Statistical significance was determined using ANOVA for multiple comparisons. When P values < 0.05, differences were deemed as the statistically significant
See this image and copyright information in PMC

References

    1. Plante JA, Liu Y, Liu J, Xia H, Johnson BA, Lokugamage KG, et al. Spike mutation D614G alters SARS-CoV-2 fitness. Nature. 2021;592:116–21. doi: 10.1038/s41586-020-2895-3. - DOI - PMC - PubMed
    1. Liu Y, Liu J, Plante KS, Plante JA, Xie X, Zhang X, et al. The N501Y spike substitution enhances SARS-CoV-2 infection and transmission. Nature 2021; 10.1038/s41586-021-04245-0. - PMC - PubMed
    1. Jhun H, Park H-Y, Hisham Y, Song C-S, Kim S. SARS-CoV-2 Delta (B.1.617.2) variant: a unique T478K mutation in receptor binding motif (RBM) of spike gene. Immune Netw. 2021;21:e32. doi: 10.4110/in.2021.21.e32. - DOI - PMC - PubMed
    1. Liu Z, VanBlargan LA, Bloyet LM, Rothlauf PW, Chen RE, Stumpf S, et al. Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization. Cell host microbe. 2021;29:477–88. doi: 10.1016/j.chom.2021.01.014. - DOI - PMC - PubMed
    1. Hu J, Gao Q, He C, Huang A, Tang N, Wang K. Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2. Genes Dis. 2020;7:551–7. doi: 10.1016/j.gendis.2020.07.006. - DOI - PMC - PubMed

Supplementary concepts