Morphological and molecular characterization of Ceratomyxa binhthuanensis n. sp. (Myxosporea: Ceratomyxidae) from the gall bladder of blacktip grouper Epinephelus fasciatus (Perciformes: Serranidae) in the East Sea of Vietnam

Nguyen Ngoc Chinh¹, Nguyen Van Ha^{2

3}, Pham Ngoc Doanh^{2

3}, Yurakhno Violetta⁴, Tomoyoshi Yoshinaga⁵, Sho Shirakashi⁶, Sascha L Hallett⁷, Christopher M Whipps⁸

Affiliations

¹ Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam. chinhnn89@gmail.com.
² Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam.
³ Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam.
⁴ A. O. Kovalevsky Institute of Biology of the Southern Seas of Russian Academy of Sciences, 2 Nakhimov Ave., 299011, Sevastopol, Russian Federation.
⁵ Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo, 112-8657, Japan.
⁶ Aquaculture Research Institute, Kindai University, 3153 Shirahama, Nishimuro, Wakayama, 649-2211, Japan.
⁷ Department of Microbiology, Oregon State University, Nash Hall 226, Corvallis, OR, 97331, USA.
⁸ SUNY-ESF, State University of New York College of Environmental Science and Forestry, Environmental and Forest Biology, 1 Forestry Drive, Syracuse, NY, 13210, USA.

PMID: 35018489
DOI: 10.1007/s00436-021-07419-x

Morphological and molecular characterization of Ceratomyxa binhthuanensis n. sp. (Myxosporea: Ceratomyxidae) from the gall bladder of blacktip grouper Epinephelus fasciatus (Perciformes: Serranidae) in the East Sea of Vietnam

Nguyen Ngoc Chinh et al. Parasitol Res. 2022 Feb.

. 2022 Feb;121(2):613-621.

doi: 10.1007/s00436-021-07419-x. Epub 2022 Jan 12.

Authors

Nguyen Ngoc Chinh¹, Nguyen Van Ha^{2

3}, Pham Ngoc Doanh^{2

3}, Yurakhno Violetta⁴, Tomoyoshi Yoshinaga⁵, Sho Shirakashi⁶, Sascha L Hallett⁷, Christopher M Whipps⁸

Affiliations

¹ Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam. chinhnn89@gmail.com.
² Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam.
³ Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi, Vietnam.
⁴ A. O. Kovalevsky Institute of Biology of the Southern Seas of Russian Academy of Sciences, 2 Nakhimov Ave., 299011, Sevastopol, Russian Federation.
⁵ Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo, 112-8657, Japan.
⁶ Aquaculture Research Institute, Kindai University, 3153 Shirahama, Nishimuro, Wakayama, 649-2211, Japan.
⁷ Department of Microbiology, Oregon State University, Nash Hall 226, Corvallis, OR, 97331, USA.
⁸ SUNY-ESF, State University of New York College of Environmental Science and Forestry, Environmental and Forest Biology, 1 Forestry Drive, Syracuse, NY, 13210, USA.

PMID: 35018489
DOI: 10.1007/s00436-021-07419-x

Abstract

A new myxozoan species, Ceratomyxa binhthuanensis n. sp. (Myxosporea: Ceratomyxidae), was found in the gall bladder of blacktip grouper Epinephelus fasciatus (Perciformes: Serranidae) in the East Sea of Vietnam. Myxospores were observed floating free in the gall bladder of 3 out of 20 fish examined (15%). Mature myxospores were elongate and slightly crescent-shaped and measured 12.2 ± 1.3 (10.8-16.0) μm in thickness and 5.8 ± 0.6 (4.8-6.9) μm in length, with two smooth equal shell valves. The two polar capsules were spherical and equal in size, measuring 2.6 ± 0.3 (2.3-2.9) μm in diameter. The posterior angle was slightly concave, 153.7° ± 5.6° (148.9°-166.0°). Molecular analysis of SSU rDNA sequence showed that Ceratomyxa binhthuanensis n. sp. differs from other Ceratomyxa spp. available in GenBank. Phylogenetic analysis indicated that C. binhthuanensis n. sp. was closely related to three species, Ceratomyxa nolani, Ceratomyxa yokoyamai, and Ceratomyxa cutmorei, which also infect fish hosts of the genus Epinephelus.

Keywords: Ceratomyxa; Molecular phylogeny; Morphology; Myxozoa; SSU rDNA.

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References

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Morphological and molecular characterization of Ceratomyxa binhthuanensis n. sp. (Myxosporea: Ceratomyxidae) from the gall bladder of blacktip grouper Epinephelus fasciatus (Perciformes: Serranidae) in the East Sea of Vietnam

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Morphological and molecular characterization of Ceratomyxa binhthuanensis n. sp. (Myxosporea: Ceratomyxidae) from the gall bladder of blacktip grouper Epinephelus fasciatus (Perciformes: Serranidae) in the East Sea of Vietnam

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