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. 2021 Oct 22;8(4):e156.
doi: 10.14440/jbm.2021.373. eCollection 2021.

Novel adaptation of a running suture technique in a mouse model of corneal transplantation

Hayate Nakagawa  1 , Tomas Blanco  1 , Francesca Kahale  1 , Rohan Bir Singh  1 , Thomas H Dohlman  1 , Reza Dana  1
Affiliations

Novel adaptation of a running suture technique in a mouse model of corneal transplantation

Hayate Nakagawa et al. J Biol Methods. .

Abstract

Several murine models of corneal transplantation have been developed over the years to study the immunopathological processes that lead to the failure of grafted corneas. In all of them, the classic eight interrupted sutures technique is utilized for transplanting the donor cornea on the host bed. However, in clinical practice, a single continuous suture with a single knot is generally performed for corneal transplantation. Here, we describe the adaptation of the single continuous suture technique in a mouse model of corneal transplantation.

Keywords: animal model; corneal transplantation; running suture technique.

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Figures

Figure 1.
Figure 1.
Schematic drawing of running suture placement. A. Mark the recipient central cornea with a 1.5 mm trephine and excise cornea. B. Place 4 intrastromal interrupted 11-0 nylon sutures to secure the donor cornea, creating 4 quadrants. C. Place running suture by taking 2 suture bites per quadrant. D. Rinse the anterior chamber with PBS. E. Adjust suture tension. F. Cut the 4 interrupted cardinal sutures using a 30 Gauge needle. G. Schematic representation of the cornea post-transplantation. H. At day 7, cut the running suture within each quadrant (green circles) and remove suture. I. Schematic representation of healed, transplanted cornea.
Figure 2.
Figure 2.
Running suture surgical procedure. A. Place 4 intrastromal interrupted 11-0 nylon sutures to secure the donor cornea to the recipient bed. B. Place continuous suture by placing 2 suture bites within each quadrant. C. Tie-off the running suture knot and remove the 4 cardinal interrupted sutures. D. Post-operative appearance immediately after surgery.
Figure 3.
Figure 3.
Representative post-operative images after corneal transplantation. A. Postoperative slit-lamp appearance 1 week after running (left) and interrupted (right) suture techniques. B. Postoperative slit-lamp appearance 8 weeks after running (left) and interrupted (right) suture techniques.
Figure 4.
Figure 4.
Anterior synechiae: a severe complication of corneal transplantation. A. Slit-lamp image showing anterior synechiae and corneal neovascularization. B. Anterior synechiae in the anterior chamber visualized on Optical Coherence Tomography.
See this image and copyright information in PMC

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