Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb:147:105063.
doi: 10.1016/j.jcv.2021.105063. Epub 2022 Jan 1.

Influence of preanalytical parameters on influenza and respiratory syncytial virus molecular detection

Affiliations

Influence of preanalytical parameters on influenza and respiratory syncytial virus molecular detection

Claire Grolhier et al. J Clin Virol. 2022 Feb.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors report no conflict of interest related to this work.All reagents were provided by Cepheid. This work was conducted independently to the manufacturer.

Figures

Fig. 1:
Fig. 1
Experimental design for each tested VTM. Fifteen positive clinical samples were analyzed: influenza A virus (n = 5), influenza B virus (n = 5), RSV-A (n = 3) or RSV-B (n = 2). Three VTM were compared: Cepheid®, Virocult® and E swab® under several temperature storage (+4 °C and ambient temperature (TA) and delay (T0, H4, H24 and H72).
Fig. 2:
Fig. 2
Comparison of dCt for each sample stored for different periods of time, under different temperature conditions and gathered according to VTM: A: Cepheid®; B: Virocult®; C: E-swab®. Each virus family is represented by a different symbol: FluA, open squares; FluB, closed squares; RSV, open circles. The dotted horizontal lines represent the 2 Ct cut-off.

References

    1. Garnett L., Bello A., Tran K.N., Audet J., Leung A., Schiffman Z., Griffin B.D., Tailor N., Kobasa D., Strong J.E. Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages. J. Virol. Methods. 2020;285 - PMC - PubMed
    1. Charlton C.L., Babady E., Ginocchio C.C., Hatchette T.F., Jerris R.C., Li Y., Loeffelholz M., McCarter Y.S., Miller M.B., Novak-Weekley S., Schuetz A.N., Tang Y.-.W., Widen R., Drews S.J. Practical guidance for clinical microbiology laboratories: viruses causing acute respiratory tract infections. Clin. Microbiol. Rev. 2018;32 - PMC - PubMed
    1. Ferroni A., Izopet J., Miedougé M. Vérification d'une méthode qualitative d'amplification génique. QUAMIC. 2019:273–282. th ed. Société Française de Microbiologie.
    1. Wolters F., Grünberg M., Huber M., Kessler H.H., Prüller F., Saleh L., Fébreau C., Rahamat-Langendoen J., Thibault V., Melchers W.J.G. European multicenter evaluation of Xpert® Xpress SARS-CoV-2/Flu/RSV test. J. Med. Virol. 2021;93:5798–5804. - PMC - PubMed
    1. Richard-Greenblatt M., Comar C.E., Flevaud L., Berti M., Harris R.M., Weiss S.R., Glaser L. Copan eNAT transport system to address challenges in COVID-19 diagnostics in regions with limited testing access. J. Clin. Microbiol. 2021;59 e00110-21. - PMC - PubMed

MeSH terms