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. 2022 Mar:59:102662.
doi: 10.1016/j.scr.2022.102662. Epub 2022 Jan 10.

Establishment of an induced pluripotent stem cell line DHMi005-A from a healthy male proband

M Dreßen  1 , T Luzius  2 , H Lahm  2 , I Neb  2 , S A Doppler  2 , S Schneider  3 , E Dzilic  2 , R Lange  4 , M Krane  5
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Free article

Establishment of an induced pluripotent stem cell line DHMi005-A from a healthy male proband

M Dreßen et al. Stem Cell Res. 2022 Mar.
Free article

Abstract

We generated an induced pluripotent stem cell (iPSC) line from a healthy male 29-year-old proband. Adipose fibroblasts (AFs) were reprogrammed using Sendai virus. Generated iPSCs showed typical stem cell morphology. From passage 9 on, iPSCs were free of virus. Pluripotency in the iPSCs was verified and spontaneous differentiation showed expression of all three germ layers. Karyotyping indicated no anomalies for the generated iPSCs. Many patient-specific iPSCs are generated from subcutaneous fat fibroblasts obtained during surgical procedure. The described control iPSC line was generated equally and therefore serves as an ideal control for adipose-fibroblast-based patient-specific iPSC lines in disease modeling.

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