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. 2022 Jan 7:2022:5297580.
doi: 10.1155/2022/5297580. eCollection 2022.

Biostatistics of VHL-Gene Transfection in the Health Informatics Analysis of Renal Cell Carcinoma

Yunxiang Gong  1 , Degang Wang  1 , Wengang Wang  1
Affiliations

Biostatistics of VHL-Gene Transfection in the Health Informatics Analysis of Renal Cell Carcinoma

Yunxiang Gong et al. Comput Math Methods Med. .

Abstract

Objective: In this paper, we study the role of the VHL gene in regulating the proliferation and apoptosis of renal cell carcinoma, as well as the safety and transfection efficiency of ultrasound microbubble gene transfection technology.

Method: We use kidney cancer cell lines as an in vitro research object and apply ultrasound microbubble gene transfection technology to transfect the VHL gene into kidney cancer cell line (786-0). The proliferation and apoptosis of cells were measured to clarify the inhibitory effect of the VHL gene in renal cell carcinoma. After that, pEGFP-VHL was transfected using ultrasonic microbubble and liposome gene transfection techniques, respectively, and the transfection efficiency was measured by immunofluorescence.

Results: Compared with untreated and 786-0 cells that are transfected with empty vector, the expression level of VHL gene mRNA in 786-0 cells that are transfected with pcDNA3.1-VHL was significantly increased, and the cell growth inhibition rate was significantly higher. The rate of apoptosis increased significantly. Transfection efficiency of the pEGFP-VHL gene after transfection of 786-0 cells for 48 h: control group 0, liposome group (35.55 ± 2.77) %, ultrasound microbubble group (18.27 ± 2.83) %, and two transfection methods on cells. There is no significant difference in the impact of vitality.

Conclusion: VHL gene expression can significantly inhibit the proliferation ability of renal cancer cell line 786-0 and promote its apoptosis. VHL gene is a potential target for gene therapy of kidney cancer.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
RT-PCR detection of VHL gene mRNA expression in 786-0 cells based on (a) control group, (b) pcDNA3.1 (+) group, and (c) pcDNA3 1-VHL group.
Figure 2
Figure 2
48 hours after transfection, the apoptotic rate of each group was detected using a loss cytometer. (a) Control group. (b) pcDNA3.1 (+) group. (c) pcDNA3 1-VHL group.
Figure 3
Figure 3
Apoptosis rate of each group 48 h after transfection.
Figure 4
Figure 4
MTT detects the activity changes of cells at 12 h, 24 h, 36 h, 48 h, 60 h, and 72 h after 786-0 transfection.
Figure 5
Figure 5
48 hours after transfection, observe the distribution of GFP fluorescence in each group under a fluorescence microscope. (a) Control group. (b) Liposome group. (c) Ultrasound microbubble group.
Figure 6
Figure 6
Comparison of transfection of empty vector with ultrasonic microbubble method and liposome method.
See this image and copyright information in PMC

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