Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr;157(4):443-457.
doi: 10.1007/s00418-021-02047-z. Epub 2022 Jan 17.

Endoplasmic reticulum stress affects mouse salivary protein secretion induced by chronic administration of an α1-adrenergic agonist

Yoko Yoshino  1   2 , Takahiro Imamura  3   4 , Shigeo Yamachika  3   4 , Tomoko Ohshima  5 , Ryoko Ushikoshi-Nakayama  6 , Hiroko Inoue  6   7 , Ichiro Saito  6 , Yoichi Nakagawa  3
Affiliations

Endoplasmic reticulum stress affects mouse salivary protein secretion induced by chronic administration of an α1-adrenergic agonist

Yoko Yoshino et al. Histochem Cell Biol. 2022 Apr.

Abstract

Stress stimulates both the sympathetic-adrenomedullary and hypothalamus-pituitary-adrenal axes. Activation of these axes results in the release of catecholamines, which in turn affects salivary secretion. Thus, repetitive stimulation of the α1-adrenergic receptor could be useful for studying the effects of chronic stress on the salivary gland. Salivary protein concentration and kallikrein activity were significantly lower in mice following chronic phenylephrine (PHE) administration. Chronic PHE administration led to significantly increased expression of the 78-kDa glucose-regulated protein, activating transcription factor 4, and activating transcription factor 6. Histological analyses revealed a decrease in the size of the serous cell and apical cytoplasm. These results suggest that repetitive pharmacological stimulation of the sympathetic nervous system elicits ER stress and translational suppression. In addition, PHE-treated mice exhibited a decrease in intracellular Ca2+ influx elicited by carbachol, a muscarine receptor agonist in the submandibular gland. The present findings suggest that chronic psychological, social, and physical stress could adversely affect Ca2+ regulation.

Keywords: Ca2+; Endoplasmic reticulum stress response; Kallikrein; Stress; α1-Adrenoceptor agonist.

PubMed Disclaimer

References

    1. Anderson LC, Garrett JR, Zhang X, Proctor GB, Shori DK (1995) Differential secretion of proteins by rat submandibular acini and granular ducts on graded autonomic nerve stimulations. J Physiol (lond) 485(Pt 2):503–511 - DOI
    1. Barka T (1980) Biologically active polypeptides in submandibular glands. J Histochem Cytochem 28(8):836–859. https://doi.org/10.1177/28.8.7003006 - DOI - PubMed
    1. Baum BJ (1987) Neurotransmitter control of secretion. J Dent Res 66:628–632 - DOI
    1. Bedi GS (1991) The effects of autonomic drugs on the concentration of kallikrein-like proteases and cysteine-proteinase inhibitor (cystatin) in rat whole saliva. J Dent Res 70(5):924–930. https://doi.org/10.1177/00220345910700051201 - DOI - PubMed
    1. Bergaya S, Meneton P, Bloch-Faure M, Mathieu E, Alhenc-Gelas F, Levy BI, Boulanger CM (2001) Decreased flow-dependent dilation in carotid arteries of tissue kallikrein-knockout mice. Circ Res 88(6):593–599 - DOI

MeSH terms

LinkOut - more resources