Streptomyces apricus sp. nov., isolated from soil

Abstract

A novel Streptomyces strain, SUN51^T, was isolated from soils sampled in Wisconsin, USA, as part of a Streptomyces biogeography survey. Genome sequencing revealed that this strain had less than 90 % average nucleotide identity (ANI) to type species of Streptomyces: SUN51^T was most closely related to Streptomyces dioscori A217^T (99.5 % 16S rRNA gene identity, 89.4 % ANI). Genome size was estimated at 8.81 Mb, and the genome DNA G+C content was 72 mol%. The strain possessed the cellular fatty acids anteiso-C_{15 : 0}, iso-C_{16 : 0}, 16 : 1 ω7c, anteiso-C_{17 : 0}, iso-C_{14 : 0} and C_{16 : 0}. The predominant menaquinones were MK-9 H4, MK-9 H6 and MK-9 H8. Strain SUN51^T contained the polar lipids phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl glycerol and diphosphatidyl glycerol. The cell wall contained ll-diaminopimelic acid. The strain could grow on a broad range of carbon sources and tolerate temperatures of up to 40 °C. The results of the polyphasic study confirmed that this isolate represents a novel species of the genus Streptomyces, for which the name Streptomyces apricus sp. nov. is proposed. The type strain of this species is SUN51^T (=NRRL B-65543^T=JCM 33736^T).

Keywords: Streptomyces; apricus; soil; species description.