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. 2022 Jan;2(1):e342.
doi: 10.1002/cpz1.342.

Cryosectioning

Mark A Ross  1 , Lauryn Kohut  2 , Patricia A Loughran  1   2
Affiliations

Cryosectioning

Mark A Ross et al. Curr Protoc. 2022 Jan.

Abstract

The protocols presented here describe steps for cryosectioning tissue samples to be used in light microscopy methodologies including histochemistry, enzyme immunohistochemistry, and immunofluorescence. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Cryosectioning.

Keywords: cryopreservation; cryosectioning and immunofluorescence; tissue perfusion-fixation.

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References

Literature Cited

    1. Fischer, A. H. (2008). Cryosectioing Tissues. Cold Spring Harbor Protocols, doi: 10.1101/pdb.prot4991.
    1. Kohut, L., Ross, M. A., & Loughran, P. A. (2022). A tissue perfusion harvest model for optimal multisystem comparisons of pathobiology. Current Protocols, 2, e343. doi: 10.1002/cpz1.343.
    1. Liyange, S., Dassanayake, R. S., Bouyanfif, A., Rajakaruna, E., Ramalingam, L., Moustaid-Moussa, N., & Abidi, N. (2017). Optimization and validation of cryostat temperature conditions for tans-reflectance mode FTIR microspectroscopic imaging of biological tissues. MethodsX, 4, 118-127. doi: 10.1016/j.mex.2017.01.006.
    1. Watkins, S. (2009). Cryosectioning. Current Protocols in Cytometry, 12.15.1-1215.7. doi: 10.1002/0471142956.cy1215s48.

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