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. 2022 Jan 13;10(1):159.
doi: 10.3390/microorganisms10010159.

Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution

Katerina Tsilipounidaki  1 , Zoi Athanasakopoulou  2 , Elke Müller  3   4 , Sindy Burgold-Voigt  3   4 , Zoi Florou  1 , Sascha D Braun  3   4 , Stefan Monecke  3   4   5 , Nikolaos K Gatselis  1 , Kalliopi Zachou  1 , Aggelos Stefos  1 , Ilias Tsagalas  1 , Marina Sofia  2 , Vassiliki Spyrou  6 , Charalambos Billinis  2   7 , George N Dalekos  1 , Ralf Ehricht  3   4   8 , Efthymia Petinaki  1
Affiliations

Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution

Katerina Tsilipounidaki et al. Microorganisms. .

Abstract

Carbapenem-resistant Gram-negative bacteria are a public health threat that requires urgent action. The fact that these pathogens commonly also harbor resistance mechanisms for several other antimicrobial classes further reduces patient treatment options. The present study aimed to provide information regarding the multidrug resistance genetic background of carbapenem-resistant Gram-negative bacteria in Central Greece. Strains from a tertiary care hospital, collected during routine practice, were characterized using a DNA microarray-based assay. Various different resistance determinants for carbapenems, other beta-lactams, aminoglycosides, quinolones, trimethoprim, sulfonamides and macrolides were detected among isolates of the same sequence type. Eighteen different multidrug resistance genomic profiles were identified among the twenty-four K. pneumoniae ST258, seven different profiles among the eight K. pneumoniae ST11, four profiles among the six A. baumannii ST409 and two among the three K. oxytoca. This report describes the multidrug resistance genomic background of carbapenem-resistant Gram-negative bacteria from a tertiary care hospital in Central Greece, providing evidence of their continuous genetic evolution.

Keywords: Acinetobacter baumannii; Greece; Klebsiella pneumoniae; Pseudomonas aeruginosa; antimicrobial resistance genes; carbapenem resistance.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Detection frequency of each resistance gene among Acinetobacter baumannii and Klebsiella pneumoniae isolates.
Figure 1
Figure 1
Detection frequency of each resistance gene among Acinetobacter baumannii and Klebsiella pneumoniae isolates.
See this image and copyright information in PMC

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